中国免疫学杂志2026，Vol.42Issue(1)：117-123,7.DOI:10.3969/j.issn.1000-484X.2026.01.018
当归多糖调节NLRP3/IL-1β信号通路对脓毒症大鼠肠黏膜损伤和免疫功能的影响
Effects of Angelica sinensis polysaccharide modulating NLRP3/IL-1β signaling pathway on intestinal mucosal injury and immune function in septic rats
摘要
Abstract
Objective:To evaluate effects of Angelica sinensis polysaccharide(ASP)on intestinal mucosal injury and immune function in septic rats and to preliminarily investigate potential mechanisms.Methods:A rat model of concentrative sepsis was pre-pared by cecum ligation and puncture(CLP)method.Rats were divided into Sham group,CLP group,low-dose ASP group(L-ASP group),medium-dose ASP group(M-ASP group),high-dose ASP group(H-ASP group),ASP+Nigericin group(ASP+N group)and Nigericin group(ASP+N group)according to randomized number table method,with 21 rats in each group.L-ASP,M-ASP and H-ASP groups were given 100,200 and 400 mg/kg ASP by gavage,respectively.ASP+N group was given 1 mg/kg NLRP3 activator Nigericin intraperitoneally on top of 400 mg/kg ASP by gavage.Equal doses of saline were given by gavage in Sham and CLP groups.Intestinal mucosal injury was assessed using HE staining and Chiu's score.Intestinal epithelial barrier function was assessed using diamine oxidase(DAO)activity assay and urinary lactose/mannitol(L/M)ratio.Serum and ileal tissue levels of IL-6,IL-1β and IL-10 were detected by ELISA.Peripheral blood T-lymphocyte subsets were detected by flow cytometry.Western blot was used to analyze intestinal epithelial barrier and NLRP3 inflammatory vesicle-associated protein expression.Results:Intestinal mucosa of CLP rats showed pathological damage such as inflammatory cell infiltration,irregular arrangement of intestinal villi,and enlarged villus gap,intestinal mucosal Chiu's score,serum DAO and urinary L/M were increased,expressions of Claudin-3,ZO-1 and occludin pro-teins were decreased in tissue of ileal mucosa,IL-6 and IL-1β levels were significantly increased,while IL-10 level was significantly decreased in serum and ileal mucosal tissues,proportion of CD4+T cells and CD4+T/CD8+T in peripheral blood were significantly de-creased.ASP treatment was able to reduce intestinal mucosal tissue damage,improve barrier function,and reduce body and intestinal immune-inflammatory responses in septic rats,reduced expressions of NLRP3,ASC and cleaved caspase-1 in intestinal tissues of sep-tic rats;while NLRP3 activator Nigericin could attenuate modulating effect of angelica polysaccharides on intestinal mucosal injury and immune-inflammatory response to some extent.Conclusion:ASP ameliorates intestinal mucosal injury,protects intestinal epithelial barrier integrity,and reduces organismal and intestinal immune-inflammatory responses in septic rats,which may be achieved through inhibition of NLRP3/IL-1β pathway activation.关键词
脓毒症/当归多糖/肠黏膜损伤/免疫功能/炎症反应/NLRP3炎症小体Key words
Sepsis/Angelica sinensis polysaccharides/Intestinal mucosal injury/Immune function/Inflammatory response/NLRP3 inflammasome分类
医药卫生引用本文复制引用
李佳,梅建强,陈分乔,王卜,张翼飞,高海运..当归多糖调节NLRP3/IL-1β信号通路对脓毒症大鼠肠黏膜损伤和免疫功能的影响[J].中国免疫学杂志,2026,42(1):117-123,7.基金项目
河北省中医药管理局科研计划项目(2024222,2024220) （2024222,2024220）
河北省自然科学基金项目(H2022423369). （H2022423369）
