中国农业科学2026，Vol.59Issue(1)：1-16,16.DOI:10.3864/j.issn.0578-1752.2026.01.001
ABC转运蛋白OsARG1调控水稻抽穗期的功能
Functions of ABC Transporter OsARG1 in Rice Heading Date Regulation
摘要
Abstract
[Objective]Heading date is a critical agronomic trait influencing rice yield and quality,regulated by complex networks involving histone-modifying enzymes,transcription factors,protein kinases,florigens,and phytochromes.While ATP-binding cassette(ABC)transporters are known for their roles in substrate transport,their functions in heading date regulation remain unclear.This study investigates the role of the ABC transporter gene OsARG1 in the regulation of rice heading date,which will provide evidence for enriching the heading date regulation network.[Method]A comparative analysis was conducted between wild-type Nipponbare and the osarg1 mutant.Key agronomic traits,including heading date,plant height,tiller number,and panicle length,were assessed.Chlorophyll contents in leaf of Nipponbare(WT),albino leaf(WL),yellow-green leaf(YL)and green leaf(GL)of osarg1 were measured.Metal element contents such as cobalt,nickel,calcium,magnesium and iron in WT,WL,YL and GL were determined by ICP-MS.Hormone profiling,transcriptome sequencing,and integrative analysis were performed on WT,WL and GL to explore the regulatory function of OsARG1.[Result]The osarg1 mutant exhibited an earlier heading date than wild-type in both Guiyang and Changchun.It also showed reduced plant height,tiller number,and panicle length.Chlorophyll levels in WL and YL were significantly lower,accompanied by disrupted metal element homeostasis.Hormonal analysis revealed elevated levels of gibberellins,auxin-related,and cytokinin-related hormones in GL and WL,particularly in WL.Transcriptome analysis identified 2 001 and 6 555 differentially expressed genes(DEGs)in GL_vs_WT and WL_vs_WT comparisons,respectively.Over 20 heading date-associated genes,including Hd3a,OsMADS14,and chromatin methyltransferase genes,were differentially expressed.GO and KEGG analyses of DEGs from GL vs WT comparison highlighted enrichment in pathways related to metabolism,development,and environmental responses.Integrated transcriptomic and hormonal analysis suggested that OsARG1 may influence gibberellin and cytokinin levels by modulating diterpene and zeatin metabolism and hormone signaling pathways.Expression levels of the selected genes by qRT-PCR were consistent with the transcriptome data,validating the transcriptomic findings.[Conclusion]The osarg1 mutant heads earlier than the wild-type,with OsARG1 likely regulating heading date through modulating the expression of heading date related genes.Additionally,OsARG1 plays roles in maintaining chlorophyll content and metal element(such as nickel,iron,and magnesium)balance in rice leaves.关键词
水稻/抽穗期/OsARG1/金属元素含量/植物激素/转录组测序Key words
rice/heading date/OsARG1/metal element content/phytohormone/transcriptome sequencing引用本文复制引用
王忠妮,雷月,李佳丽,宫彦龙,朱速松..ABC转运蛋白OsARG1调控水稻抽穗期的功能[J].中国农业科学,2026,59(1):1-16,16.基金项目
国家自然科学基金(32260496)、贵州省基础研究(自然科学)项目(黔科合基础-ZK[2023]一般177) （32260496）
