中国兽医杂志2026，Vol.62Issue(1)：79-84,6.DOI:10.20157/j.cnki.zgsyzz.2026.01.010
鸭瘟病毒野毒株的分离鉴定和疫苗免疫试验
Isolation,Identification and Vaccine Immunity Testing of a Wild Type Duck Plague Virus Strain
摘要
Abstract
To determine whether the outbreak in Cherry Valley breeder ducks in a farm in Jiangsu Province in January 2024 was related to a variant of duck plague virus,tissues from the liver,spleen,intestine,esophagus,and trachea of dead ducks were collected for virus isolation and culture.The isolated strain was subjected to PCR amplification based on the unique long region 2(UL2)gene and long open reading frame 5(LORF5)gene,as well as animal regression experiments,virulence determination,and vaccine immunization evaluation.The results showed that one duck plague virus strain was successfully isolated and designated QL240109.The UL2 gene fragment length of this strain was consistent with that of the attenuated strain,whereas the LORF5 gene fragment length was consistent with that of the virulent strain.Animal regression experiments demonstrated that the isolated strain was highly lethal to susceptible Cherry Valley ducks.Virulence determination indicated that the strain had lower virulence than the classical Chinese virulent strain CVCC AV1221.Vaccine efficacy evaluation revealed that the duck plague live vaccine provided 75％-100％ protection against the isolated strain.These findings suggest that,compared with the attenuated vaccine strain and classical virulent strains in China,the isolated strain underwent genomic variation,exhibited strong lethality but lower virulence than classical virulent strains,and could still be effectively controlled by duck plague live vaccines.The results provide a reference for the prevention and control of this mutant strain of duck plague virus in duck farms.关键词
鸭瘟病毒/流行毒株/分离鉴定/疫苗免疫Key words
duck plague virus/epidemic strain/isolation and identification/vaccine immunity分类
农业科技引用本文复制引用
张连秀,贾爱琴,宋晓飞,彭建云,李振,汪建华,许明清,李富金..鸭瘟病毒野毒株的分离鉴定和疫苗免疫试验[J].中国兽医杂志,2026,62(1):79-84,6.基金项目
山东省重点研发计划(重大科技创新工程)(2023CXGC010704) （重大科技创新工程）
