中国实验动物学报2025，Vol.33Issue(12)：1739-1748,10.DOI:10.3969/j.issn.1005-4847.2025.12.003
3D打印个性化钛网联合GBR对兔ORNJ骨缺损修复效果的影响
Effect of 3D-printed individualized titanium mesh plus guided bone regeneration on jaws osteoradionecrosis bone defects in rabbits
摘要
Abstract
Objective To investigate the efficacy of 3D-printed individualized titanium mesh(3D-PITM)combined with guided bone regeneration(GBR)in a rabbit model of osteoradionecrosis of the jaws(ORNJ)with bone defects.Methods Eighteen white rabbits with ORNJ-induced bone defects were divided randomly into three groups:a group(debridement and suturing for natural healing),b group(debridement and conventional GBR),and c group(debridement and 3D-PITM-supported GBR).Two rabbits from each group were euthanized at 4,8,and 12 weeks,respectively.Bone defect repair was evaluated by immunohistochemical staining to detect expression levels of the osteogenesis-related factors alkaline phosphatase(ALP),osteocalcin(OC),and bone morphogenetic protein-2(BMP-2),and the inflammatory factors interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α).Results There was no significant difference in ALP expression among the groups at 4 weeks,but OC levels were lower in c group than in b group.At 8 and 12 weeks,however,ALP,BMP-2,and OC expression levels were all significantly higher in c group than in a and b groups.Expression levels of IL-6 and TNF-α were significantly lower in c group than in Groups a and b at 4,8,and 12 weeks,but there was no significant difference in IL-6 expression levels between groups b and c at 4 weeks.Conclusions The combination of 3D-PITM and GBR demonstrated significantly superior bone defect repair efficacy in ORNJ compared with conventional GBR or natural healing alone.关键词
颌骨放射性骨坏死/3D打印个性化钛网/碱性磷酸酶/骨形成蛋白2/骨钙素/白细胞介素-6/肿瘤坏死因子-αKey words
osteoradionecrosis of jaws/3D printing individualized titanium mesh/alkaline phosphatase/bone-morphogenetic protein-2/osteocalcin/interleukin-6/tumor necrosis factor-α分类
生物科学引用本文复制引用
赵弼洲,田佳灵,党晓兵,胡永寿,马海冰..3D打印个性化钛网联合GBR对兔ORNJ骨缺损修复效果的影响[J].中国实验动物学报,2025,33(12):1739-1748,10.基金项目
甘肃省科技计划(23JRRA1232). Funded by Science and Technology Project of Gansu Province(23JRRA1232). （23JRRA1232）
