中国实验动物学报2025，Vol.33Issue(12)：1772-1783,12.DOI:10.3969/j.issn.1005-4847.2025.12.006
肺炎支原体和流感病毒H1N1共感染动物模型实验研究
Experimental study on animal models coinfection of Mycoplasma pneumoniae and influenza virus H1N1
摘要
Abstract
Objective To establish an animal model of co-infection with Mycoplasma pneumoniae and influenza virus H1N1.The aim is to clarify the co-pathogenic mechanism of co-infection of the two pathogens and provide a theoretical basis for the clinical intervention of mixed respiratory tract infections.Methods Twenty-four Syrian hamsters were divided into four groups:control(C)group,infection group(H1N1 influenza virus single-infection(Flu)group,Mycoplasma pneumoniae single-infection(MP)group,and Mycoplasma pneumoniae and H1N1 influenza virus co-infection(F+M)group).The animals were challenged via intranasal drops and tracheal injection.Clinical indicators were recorded for 14 d,physiological parameters and pathogen shedding were monitored,and animals were selected on days 7 and 14 post-infection for histopathological examination,viral antigen detection,and nucleic acid testing.Results Syrian hamsters in Flu,MP and F+M group exhibited clinical symptoms similar to human influenza or Mycoplasma pneumoniae infections.Nucleic acids of both pathogens were detected in nasal swabs,throat swabs,anal swabs,blood,and tissues.Histopathological result showed varying degrees of pathological damage in different tissues of the infection groups.Conclusions The clinical symptoms,viral replication,and pathological manifestations confirm the successful establishment of a Syrian hamster model co-infected with Mycoplasma pneumoniae and influenza virus H1N1.This model provides an important experimental foundation for elucidating the mechanisms of respiratory co-infections,as well as for vaccine and drug development and clinical treatment strategies.关键词
叙利亚仓鼠/呼吸道传染疾病/肺炎支原体/流感病毒H1N1/动物模型Key words
Syrian hamster/respiratory infectious diseases/Mycoplasma pneumoniae/influenza virus H1N1/animal model分类
生物科学引用本文复制引用
马骏驰,吴雅楠,和占龙,赵远,靳玮华,李艳艳,解学谦,李艾亦,杨凤梅,段素琴,唐洁,李明学..肺炎支原体和流感病毒H1N1共感染动物模型实验研究[J].中国实验动物学报,2025,33(12):1772-1783,12.基金项目
国家重点研发计划(2024YFF0728801),中国医学科学院医学与健康科技创新工程(2021-I2M-1-024). Funded by National Key Research and Development Program of China(2024YFF0728801),CAMS Innovation Fund for Medical Sciences(2021-I2M-1-024). （2024YFF0728801）
