中国农业大学学报2026，Vol.31Issue(1)：59-72,14.DOI:10.11841/j.issn.1007-4333.2026.01.06
不同砧木嫁接对番茄幼苗耐旱性的影响
Effects of different rootstock grafting on drought tolerance of tomato plants
摘要
Abstract
To investigate the effect of grafting technology on alleviating drought stress in tomato,cherry tomato'Jingfan Chuanhong No.1'was used as the scion,and'Torubam','Qiezhen3','Fanzhen2',and self-grafted cherry tomato were used as rootstocks.Through natural drought treatment,the growth performance,photosynthetic characteristics,osmotic adjustment capacity,antioxidant system,and related gene transcription under drought stress were systematic conducted.The results showed that:1)Drought stress significantly inhibited tomato seedling growth,reduced net photosynthetic rate(Pn)and stomatal conductance(Gs),and induced membrane lipid peroxidation and reactive oxygen species(ROS)accumulation.2)The type of rootstock significantly influenced the drought tolerance of grafted plants.The performance of'Torubam'rootstockgrafted plantswas the best.Compared with self-grafted plants under normal water supply,the total chlorophyll content and carotenoid content of'Torubam'-grafted plants decreased by 17.9％,while the activities of SOD and POD increased by 120％and 285.6％,respectively.The contents of proline,soluble sugar,and malondialdehyde(MDA)increased by 58.6％,41.1％,and 83.3％,respectively.Although'Qiezhen 3'and'Fanzhen 2'provided some drought tolerance advantages,their effects were weaker than'Torubam'.3)Gene expression analysis indicated that grafted plants with'Torubam'rootstock can better maintain the expression levels of genes related to photosynthetic pigment synthesis(GSA,PBGD,PSY2,ZDS)and aquaporin-related genes(PIP1.2,PIP1.5,PIP2.1,PIP2.4),while coordinately upregulating the expression of antioxidant enzyme genes(SISOD1,SISOD2,SIPOD42,SIPOD17),thereby significantly enhancing the overall antioxidant defense capacity of tomato seedlings.This study reveals the physiological and molecular differences among rootstocks in regulating tomato drought tolerance,providing a theoretical basis and practical reference for rootstock selection and efficient utilization in drought-prone areas for tomato cultivation.关键词
砧木筛选/樱桃番茄/生理机制/抗氧化防御/基因表达Key words
rootstock screening/Cherry tomato/physiological mechanisms/antioxidant defense/gene expression分类
农业科技引用本文复制引用
张子怡,陈婕,季延海,刘明池,梁浩,王星..不同砧木嫁接对番茄幼苗耐旱性的影响[J].中国农业大学学报,2026,31(1):59-72,14.基金项目
北京市农林科学院科技创新能力建设专项(KJCX20240407、KJCX20251305) （KJCX20240407、KJCX20251305）
财政部和农业农村部:国家现代农业产业技术体系资助项目(CARS-23-G-06) （CARS-23-G-06）
北京市农林科学院蔬菜研究所茄果类协同创新中心建设项目(XTCX202303) （XTCX202303）
