中国农业大学学报2026，Vol.31Issue(1)：103-114,12.DOI:10.11841/j.issn.1007-4333.2026.01.09
粪菌移植对肉鸡肠炎的抗炎作用及肠道健康调控研究
Anti-inflammatory effects and gut health regulation of fecal microbiota transplantation in broilers
摘要
Abstract
To investigate the regulatory effects of fecal microbiota transplantation(FMT)on intestinal inflammation,barrier function,and microbial homeostasis in broilers,160 healthy 1-day-old yellow-feathered broilers were selected and raised until 19 days of age.They were randomly divided into four groups:saline control group(CT),intestinal inflammation model group(CD),low-dose FMT group(DFL),and high-dose FMT group(DFH),with 40 chickens per group,over a 46-day experimental period.Body weight,average daily gain and feed conversion ratio were statistically analyzed across groups.Serum biochemical parameters were measured by ELISA,while jejunal tight junction proteins and TLR4/NF-κB pathway gene expression were analyzed using quantitative real-time PCR(qRT-PCR).Histopathological evaluation was performed to assess intestinal structural changes and pathological damage.Metagenomic sequencing was employed to characterize microbial diversity.The results showed that:1)FMT significantly alleviated growth inhibition in enteritis broilers.Compared to the CD group,both the DFL and DFH groups exhibited significantly higher body weights at 39 and 46 days of age and elevated the average daily gain(ADG)from days 32 to 46(P＜0.05).2)FMT suppressed intestinal inflammation and restored barrier function.After FMT intervention,compared with the CD group,the levels of pro-inflammatory factors IL-1β and TNF-α in the DFL group and DFH group were significantly decreased(P＜0.05).In the DFL group,jejunal villus height increased(P＜0.05),crypt depth decreased(P＜0.05).In the DFH group,tight junction proteins Claudin-1 and ZO-1 gene expression were upregulated(P＜0.05),and TLR4/NF-κB pathway gene expression was inhibited(P＜0.05)in the DFL group.3)FMT reshaped microbial homeostasis.Metagenomic analysis showed that the cecal microbial ACE index of the DFL and DFH group was significantly improved compared with the CD group(P＜0.05).Anosim analysis demonstrated significant separation between FMT and CD microbial communities(R=0.326 8,P＜0.05).In conclusion,FMT may alleviate the impacts enteritis on broilers through multi-target regulation,including modulating the TLR4/NF-κB inflammatory pathway,restoring the intestinal barrier,and remodeling microbiota homeostasis.This study provides a theoretical basis for the application of FMT in poultry production and for an alternative to antibiotics in treating enteritis broilers.关键词
粪菌移植/肉鸡/肠炎/生长性能/肠道屏障/盲肠微生物Key words
fecal microbiota transplantation/broilers/intestinal inflammation/growth performance/intestinal barrier/cecal microbiota分类
农业科技引用本文复制引用
陈家磊,熊柱翔,汪江先,杜龙环,余春莲,余春林,邱莫寒,杨朝武,朱师良,彭涵,杨礼,胡陈明,熊霞,张增荣,夏波,宋小燕..粪菌移植对肉鸡肠炎的抗炎作用及肠道健康调控研究[J].中国农业大学学报,2026,31(1):103-114,12.基金项目
四川省基本科研业务专项(SASA202401) （SASA202401）
现代农业产业体系项目(CARS-41-G07) （CARS-41-G07）
鸡创新团队(SCCXTD-2025-24) （SCCXTD-2025-24）
四川省重点研发项目(2021YFYZ0031) （2021YFYZ0031）
现代畜牧业专项项目(SASA2025CZYX002) （SASA2025CZYX002）
