中国中医急症2025，Vol.34Issue(12)：1836-1842,7.DOI:10.3969/j.issn.1004-745X.2025.12.005
溃结宁膏穴位敷贴调控内质网应激介导的IRE1α/JNK通路对溃疡性结肠炎大鼠结肠上皮细胞凋亡的影响
Exploring the Impact of Kuijiening Ointment Acupoint Application on Colonic Epithelial Cells in Ulcerative Colitis Rats Based on Endoplasmic Reticulum Stress-Mediated IRE1α/JNK Apoptotic Pathway
摘要
Abstract
Objective:To observe the therapeutic effect of Kuijiening Ointment acupoint application on ulcer-ative colitis(UC)rats under a state of"deficiency and stasis"and its impact on the endoplasmic reticulum stress(ERS)-mediated IRE1α/JNK pathway,thereby exploring the mechanism of action of this treatment for UC.Meth-ods:SD rats were randomly divided into a blank group(n=10)and a modeling group(n=50).The UC model un-der a state of"deficienoy and stasis"was induced using adenine and senna leaf gavage followed by 2,4,6-trinitro-benzene sulfonic acid enema.After modeling,the rats were further divided based on body weight into model group,non-acupoint application group,acupoint without application group,acupoint application group,and salicylazosulfa-pyridine(SASP)group,each receiving corresponding treatments for 2 weeks.Disease Activity Index(DAI)and Co-lon Mucosal Damage Index(CMDI)scores were calculated.Histopathological changes in colon tissues were ob-served after HE staining.ELISA was used to measure FasL and IL-8 levels in colon tissue homogenates.TUNEL assay was performed to determine the apoptosis rate of colonic epithelial cells.Western blotting was used to detect protein expression levels of GRP94,p-IRE1α,p-JNK,Bax,Bcl-2,and Cleaved Caspase-3 in colon tissues.Re-sults:Compared with the blank group,the model group showed reduced body weight,irregular colonic mucosa mor-phology,significant ulcers,and inflammatory cell infiltration.DAI and CMDI scores increased(P＜0.01),as were FasL and IL-8 levels in colon tissue homogenates(P＜0.01).Apoptosis rates of colonic epithelial cells increased(P＜0.01),along with elevated protein expression levels of GRP94,p-IRE 1α,p-JNK,Bax,and Cleaved-Caspase-3(P＜0.01)and decreased BCL2 protein expression(P＜0.01).Compared with the model,non-acupoint application,and acupoint without application groups,both the acupoint application group and the SASP group exhibited in-creased body weight,reduced pathological damage to the colonic mucosa,lower DAI and CMDI scores(P＜0.01),decreased FasL and IL-8 levels(P＜0.01),reduced apoptosis rates(P＜0.01),and downregulated protein expres-sion levels of GRP94,p-IRE 1α,p-JNK,Bax,and Cleaved Caspase-3(P＜0.01),with upregulated Bcl-2 protein expression(P＜0.01).There were no statistically significant differences between the acupoint application group and the SASP group in terms of various scores and apoptosis rates(P＞0.05).Conclusion:Kuijiening Ointment acupoint application may treat UC by regulating the ERS-mediated IRE1α/JNK pathway,reducing excessive apop-tosis of intestinal epithelial cells.关键词
溃疡性结肠炎/溃结宁膏/穴位敷贴/IRE1α/JNK通路/凋亡/内质网应激/大鼠Key words
Ulcerative colitis/Kuijiening Ointment/Acupoint application/IRE1α/JNK pathway/Apoptosis/Endo-plasmic reticulum stress/Rats分类
医药卫生引用本文复制引用
黄秀山,王璇,周滔,张伟,朱莹..溃结宁膏穴位敷贴调控内质网应激介导的IRE1α/JNK通路对溃疡性结肠炎大鼠结肠上皮细胞凋亡的影响[J].中国中医急症,2025,34(12):1836-1842,7.基金项目
国家自然科学基金项目(82374426) （82374426）
全国名老中医药专家传承工作室建设项目(国中医药人教函[2022]75号) （国中医药人教函[2022]75号）
湖南省中医药科研课题(D2022096) （D2022096）
湖南省中医药大学研究生科研创新课题(2024CX206) （2024CX206）
