中国中医急症2025，Vol.34Issue(12)：1854-1857,1866,5.DOI:10.3969/j.issn.1004-745X.2025.12.008
β-石竹烯干预脑缺血再灌注损伤大鼠认知功能障碍的分子机制研究
Molecular Mechanisms of β-Caryophyllene in Alleviating Cognitive Dysfunction Induced by Cerebral Ischemia-Reperfusion in Rats
摘要
Abstract
Objective:To explore the neuroprotective effect of β-caryophyllene(BCP)on cognitive impairment induced by cerebral ischemia-reperfusion injury(CIRI)through activating cannabinoid CB2 receptor.Methods:A total of 120 SPF-grade male Sprague-Dawley(SD)rats were used to establish the middle cerebral artery occlu-sion(MC AO)model,followed by 2 hours of ischemia and 24 hours of reperfusion.The rats were divided into sham operation group,solvent group,CB2 antagonist(AM 630,3 mg/kg)group,and BCP groups with high(200 mg/kg),medium(100 mg/kg)and low(50 mg/kg)doses.Intraperitoneal injection was administered continuously for 4 weeks after surgery.Results:Behavioral tests including Morris water maze and step-down test showed that com-pared with the model group,each BCP dose group had significantly shortened escape latency(P＜0.05),increased number of platform crossings(P＜0.05),prolonged step-down latency(P＜0.05)and decreased number of errors(P＜0.05),indicating that BCP could improve behavioral indicators(P＜0.05).Molecular mechanism studies re-vealed that the protein expressions of Nrf2 and PPARγ in hippocampal tissues were increased(P＜0.05)in BCP groups,while the expressions of inflammation-related factors COX-2 and NF-κB were decreased(P＜0.05).Con-clusion:BCP activates CB2 receptor and coordinately regulates the Nrf2 antioxidant pathway and PPARγ/NF-κB anti-inflammatory signaling axis,thereby alleviating oxidative stress and neuroinflammation,and improving cogni-tive impairment induced by CIRI.This study provides a potential target for clinical treatment.关键词
认知功能障碍/脑缺血再灌注损伤/β-石竹烯/CB2激动剂/大鼠Key words
Cognitive impairment/Cerebnal ischemia-reperfusion injary/β-caryophyllene/CB2 agonist/Rats分类
医药卫生引用本文复制引用
李锐淇,李卓恒,肖智予,徐露..β-石竹烯干预脑缺血再灌注损伤大鼠认知功能障碍的分子机制研究[J].中国中医急症,2025,34(12):1854-1857,1866,5.基金项目
重庆市教委科学技术研究项目(KJQN202202806) （KJQN202202806）
