作物研究2025，Vol.39Issue(6)：516-522,7.
玉竹根尖愈伤组织诱导和增殖研究
Callus Induction and Proliferation of Polygonatum odoratum Root Tips
摘要
Abstract
Polygonatum odoratum(Mill.)Druce is a significant medicinal and edible herb.Traditional asexual propagation methods face challenges including slow sprouting,low propagation rates,continuous cropping obstacles,and cultivar degradation.The difficulty of in vitro regeneration further restricts its genetic improvement and industrial application.To establish an efficient and stable system for callus induction and proliferation,this study used the root tips of Polygonatum odoratum as explants.Different sterilization treatments and medium formulations were designed to identify the optimal conditions for rhizome disinfection,root induction,as well as callus induction and proliferation.The results indicated that the optimal disinfection protocol was treatment with 75%ethanol for 80 s followed by 0.1%HgCl2 for 40 min,reducing contamination rate to 47%while maintaining explant viability.MS medium supplemented with 1.0 mg/L NAA was most effective for rooting,producing the maximum number and length of new roots from rhizomes after 16 days of dark culture.Callus induction was most effective on MS medium containing 1.5 mg/L 2,4-D and 0.5 mg/L 6-BA,achieving 93.52%induction with 16 days.For callus proliferation,MS medium with 0.5 mg/L 2,4-D and 2.0 mg/L 6-BA was most effective,yielding a proliferation coefficient of 11.52 after 50 days in dark culture,with callus appeared pale yellow,compact,and nodular in morphology with strong differentiation capacity.In contrast,elevated 2,4-D concentrations suppressed proliferation and induced browning.This work presents the first efficient root tip-based callus induction and proliferation system for P.odoratum,characterized by a high induction rate,short cycle,strong proliferation,and significant potential for virus elimination.The established system provides a technical foundation for virus-free micropropagation,germplasm conservation,and genetic transformation studies of polygonatum odoratum,contributing to its broader industrial application.关键词
玉竹/繁殖/组织培养/愈伤组织/脱毒苗/再生体系Key words
Polygonatum odoratum/propagation/tissue culture/callus/virus-free seedling/regeneration system分类
农业科技引用本文复制引用
唐艳梅,谢进,孙梦姗,周利,肖六军,胡政辉,宋荣..玉竹根尖愈伤组织诱导和增殖研究[J].作物研究,2025,39(6):516-522,7.基金项目
湖南省农业科技创新资金项目(2024CX46) （2024CX46）
湖南省中药材产业技术体系项目(HARS-11) （HARS-11）
郴州国家可持续发展议程创新示范区省级专项项目(2024sfq16). （2024sfq16）
