作物研究2025，Vol.39Issue(6)：531-539,9.
外源海藻糖对烤烟幼苗低温耐受性的影响
Effects of Exogenous Trehalose on Low Temperature Tolerance of Flue-Cured Tobacco Seedlings
摘要
Abstract
To explore the effect of exogenous trehalose solution on the low-temperature tolerance of flue-cured tobacco seedlings,the seedlings of the flue-cured tobacco variety Xiangyan No.7(at the 6-8 leaf stage)were used as the experimental materials.A controlled experiment design was adopted,with distilled water treatment and trehalose treatment set up.200 mL of distilled water and 15 mM of trehalose solution were applied externally for 24 hours,and then transferred to a light incubator for treatment at 25℃normal temperature and 4℃low temperature stress for 2 days.The phenotype was observed and analyze the changes in physiological and biochemical indices as well as the expression of stress-resistant genes.Phenotypic analysis showed that exogenous application of trehalose significantly alleviatedthe growth inhibition of tobacco caused by low temperature stress.Physiological and biochemical assay results showed that exogenous trehalose application significantly reduced malondialdehyde(MDA),relative electrical conductivity(EL),superoxide anion(OFR)and hydrogen peroxide(H2O2)contents of tobacco under low temperature stress;obviouslyincreased the activities of ascorbate peroxidase(APX),peroxidase(POD)glutathione(GSH)and catalase(CAT),increased contents of osmotic adjustment substance proline(PRO)and soluble protein(SP).Results of gene expression revealed that exogenous trehalose significantly upregulated the expression levels of these stress-responsive genes such as ERDs,CORs,RAB28,RD29A,and RD19 in tobacco seedlings under low-temperature stress.Comprehensive analysis shows that exogenous application of trehalose can enhance the tolerance of tobacco seedlings to low-temperature stress.关键词
烤烟/幼苗/海藻糖/低温胁迫/生理生化特性/基因表达Key words
flue-cured tobacco/seeding/trehalose/low temperature stress/physiological and biochemical characteristics/gene expression分类
农业科技引用本文复制引用
尹慧,王月,胡日生,户正荣,任晓敏,姜习振,李洋洋,向世鹏,黎修平,唐浩存,朱露萍,王建林..外源海藻糖对烤烟幼苗低温耐受性的影响[J].作物研究,2025,39(6):531-539,9.基金项目
中国烟草总公司科技项目(110202103011 （）
110202201012) （）
中国烟草总公司湖南省公司科技项目(HN2024KJ02). （HN2024KJ02）
