中南民族大学学报（自然科学版）2026，Vol.45Issue(2)：168-173,6.DOI:10.20056/j.cnki.ZNMDZK.20250835
不同产地牛舌草对皮质酮诱导HT-22细胞损伤的神经保护作用
Neuroprotective effect of Anchusa italica from different habitats on HT-22 cell injury induced by corticosterone
摘要
Abstract
Based on the traditional clinical efficacy and modern pharmacological research of Anchusa italica Retz.,the pharmacological effects and material basis of Anchusa italica Retz.were studied systematically by constructing an in vitro depression model.The chemical constituents of total flavonoid of Anchusa italica(TFAI)from different habitats(Xinjiang,Pakistan,Hainan)were detected by high performance liquid chromatography(HPLC).The hippocampal cells(HT-22)were used as the research object,and the nerve cell injury model was induced by corticosterone.The effects of five kinds of Anchusa italica Retz.on the viability of HT-22 cells were detected by MTT method,and the EC50 was calculated.The results showed that TFAI from different habitats had no obvious cytotoxic effect on HT-22 cells at a concentration of 500 µg/mL,and had a significant proliferation effect on HT-22 cells,and had a significant cytoprotective effect on hippocampal cell damage induced by excessive corticosterone.Compared with the flavonoid content and EC50 value of 5 batches of Anchusa italica Retz.,the flavonoid content in Anchusa italica Retz.from Hainan was the highest,and its EC50 value on HT-22 cell proliferation was the lowest,with a high effect value.The results show that the five batches of TFAI extracts have a protective effect on corticosterone-induced HT-22 hippocampal cell injury,which can increase the viability of HT-22 cells to varying degrees and have a significant neuroprotective effect.关键词
意大利牛舌草/抑郁症/HT-22细胞/神经保护Key words
Anchusa italica Retz./depression/HT-22 cell/neuroprotection分类
医药卫生引用本文复制引用
丑凌芸,崔琴丹,李竣,陶浩,黄先菊,张曌..不同产地牛舌草对皮质酮诱导HT-22细胞损伤的神经保护作用[J].中南民族大学学报（自然科学版）,2026,45(2):168-173,6.基金项目
湖北省重点研发计划项目(2024BBB033) （2024BBB033）
新疆维吾尔自治区自然科学基金资助项目(2022D01F44) （2022D01F44）
中南民族大学学术创新团队经费项目资助(XTZ24025) （XTZ24025）
湖北省国际合作基地(SH2311) （SH2311）
