中国水稻科学2026，Vol.40Issue(1)：37-50,14.DOI:10.16819/j.1001-7216.2026.250202
OsERF93参与调控水稻纹枯病抗性的研究
Function of OsERF93 in Regulating Resistance to Sheath Blight in Rice
摘要
Abstract
[Objective]This study aims to elucidate the function and mechanism of the ERF transcription factor OsERF93 in rice sheath blight resistance,providing genetic resources for disease-resistant breeding.[Method]The expression pattern,transcriptional regulatory function,and subcellular localization of OsERF93 were analyzed using RT-qPCR and transcriptional activation assays in rice protoplasts.Transgenic overexpression and knockout lines were constructed to investigate the role of OsERF93 in sheath blight resistance.Expression differences of salicylic acid(SA),jasmonic acid(JA),and ethylene(ET)-related signaling genes were compared before and after inoculation in transgenic lines.Additionally,agronomic traits of overexpression lines and controls were evaluated.[Result]OsERF93 was highly expressed in rice leaves and leaf sheaths and induced by sheath blight fungus infection.The encoded protein localized to the nucleus and cell membrane,exhibiting transcriptional activation activity.Overexpression of OsERF93 significantly enhanced sheath blight resistance,while knockout lines showed reduced resistance.JA biosynthesis gene OsAOS2 and certain PR genes were upregulated in overexpression lines and downregulated in knockout lines.Overexpression lines exhibited slightly reduced plant height and thousand-grain weight compared to wild-type controls but had significantly higher grain number per panicle.No significant differences were observed in tiller number,primary branch number,and panicle length between overexpression lines and controls.[Conclusion]The results indicate that OsERF93 positively regulates rice sheath blight resistance by activating the jasmonic acid signaling pathway,offering a novel genetic resource for rice disease-resistant breeding.关键词
水稻/纹枯病/OsERF93/茉莉酸/农艺性状Key words
rice/sheath blight/OsERF93/jasmonic acid/agronomic trait引用本文复制引用
岳轩宇,谢文亚,冯志明,陈宗祥,胡珂鸣,左示敏..OsERF93参与调控水稻纹枯病抗性的研究[J].中国水稻科学,2026,40(1):37-50,14.基金项目
国家自然科学基金资助项目(32272110,32200430) （32272110,32200430）
生物育种钟山实验室项目(ZSBBL-KY2023-06-3) （ZSBBL-KY2023-06-3）
扬州市现代农业项目(YZ2024045). （YZ2024045）
