中国水稻科学2026，Vol.40Issue(1)：72-84,13.DOI:10.16819/j.1001-7216.2026.241209
聚合稻瘟病抗性基因Pigm-1和Pid2的水稻三系不育系福梦A的选育与利用
Breeding and Utilization of the Three-Line Male Sterile Line Fumeng A with Pyramided Rice Blast Resistance Genes Pigm-1 and Pid2
摘要
Abstract
[Objective]Rice blast is one of the most important diseases in rice production and poses a serious threat to global rice production.The large-scale and long-term planting of varieties with a single resistance gene often leads to the loss of resistance.Molecular marker-assisted selection is one of the most effective approaches for controlling rice blast.[Method]Using Taifeng B as the recipient,and Shuangkang 77009 carrying Pigm-1 and Digu B carrying Pid2 as donors,respectively.The genotypes of each generation were analyzed and identified by means of hybridization,backcrossing,backcrossing and recurred cross,aided by molecular marker-assisted breeding.Subsequently,the blast resistance of the developed sterile line Fumeng A and its hybrids was evaluated using 12 blast isolates from diverse sources under natural field conditions.Finally,through field trials,the fertility,main agronomic traits,combining ability,and heterosis of Fumeng A were systematically analyzed,and the photo-sensitive characteristics of its corresponding restorer line were also examined.[Result]A new three-line male sterile line,Fumeng A,carrying both Pigm-1 and Pid2,was developed.Inoculation tests both in the laboratory and field confirmed its blast resistance.Fertility identification showed that both the sterility rate and sterility degree of Fumeng A reached 100%,with a stigma exsertion rate of 57.39%.Genetic background and agronomic trait analysis indicated that Fumeng A inherited the main agronomic traits of the recurrent parent and exhibited improved yield potential.Heterosis analysis revealed that half of the hybrid combinations derived from Fumeng A yielded higher than the control variety Tianyouhuazhan,demonstrating good potential for production application.[Conclusion]Using molecular marker-assisted selection,a novel three-line male sterile line,Fumeng A(B),with high blast resistance,stable sterility,high stigma exsertion,and excellent agronomic and yield traits was developed.This work achieved targeted improvement of blast resistance in a sterile line and created a new parental material for three-line hybrid rice breeding.关键词
水稻/抗稻瘟病/Pigm-1/三系不育系/聚合育种/抑制感光Key words
rice/resistance to rice blast/Pigm-1/three-line sterile line/polymerization breeding/photoinhibition引用本文复制引用
程朝平,何旎清,白康呈,林少俊,黄凤凰,刘军化,程祖锌,黄成志,杨德卫..聚合稻瘟病抗性基因Pigm-1和Pid2的水稻三系不育系福梦A的选育与利用[J].中国水稻科学,2026,40(1):72-84,13.基金项目
福建省公益项目(2024R1022001,2023R1021006) （2024R1022001,2023R1021006）
福建省竞争性公益项目(2024R1055) （2024R1055）
国家自然科学基金资助项目(32402387) （32402387）
5511协同工程项目(XTCXGC2021001) （XTCXGC2021001）
福建省农业科学院对外合作项目(DKBF-2024-12) （DKBF-2024-12）
福建省自然科学基金资助项目(2022J01450 （）
2024J01171). （）
