浙江农业科学2026,Vol.67Issue(1):47-51,5.DOI:10.16178/j.issn.0528-9017.20250657
连云港卷丹百合组织培养技术初探
Preliminary study on tissue culture technology of Lilium lancifolium Thunb.in Lianyungang
封士达 1葛金涛 1金东林 2徐艳 1王多明 1邵小斌1
作者信息
- 1. 连云港市农业科学院,江苏 连云港 222000
- 2. 江苏海洋大学,江苏 连云港 222000
- 折叠
摘要
Abstract
Lilium lancifolium Thunb.is a special type of lily used in the Yuntai Mountains of Lianyungang.Due to thefts by nearby residents and gnawing by cattle and sheep,the species are on the verge of extinction.In order to establish a rapid breeding technology system,this study used scales of Lilium lancifolium Thunb.as explants.It was found that the disinfection treatment of treating scales with 75%ethanol for 30 seconds and 2%NaClO for 10 minutes was more appropriate.The appropriate culture medium for induction of adventitious buds was MS+45 g·L-1 sucrose+0.1 mg·L-1 NAA+1.0 mg·L-1 6-BA.The appropriate culture medium for rooting of adventitious buds was MS+0.3 mg·L-1NAA.关键词
卷丹百合/组织培养/不定芽/培养基Key words
Lilium lancifolium Thunb./tissue culture/adventitious bud/culture medium分类
农业科技引用本文复制引用
封士达,葛金涛,金东林,徐艳,王多明,邵小斌..连云港卷丹百合组织培养技术初探[J].浙江农业科学,2026,67(1):47-51,5.