中国水稻科学2026，Vol.40Issue(1)：85-94,10.DOI:10.16819/j.1001-7216.2026.240718
水稻类病变早衰突变体lmes7的鉴定与基因精细定位
Phenotypic Identification and Gene Fine Mapping of the Lesion Mimic and Early Senescence Mutant lmes7 in Rice
摘要
Abstract
[Objective]To study the molecular mechanisms of defense response and leaf premature senescence in rice,[Method]we screened a lesion mimic and early senescence mutant,designated lmes7(lesion mimic and early senescence 7),from a mutant library of the japonica rice variety Wuyunjing 7.The main agronomic traits of the mutant and the photosynthetic pigment content in its leaves were measured.The target gene was identified through fine mapping and sequencing,and its encoded protein was analyzed and aligned.[Result]Physiological analysis showed that the photosynthetic pigment content in the mutant leaves was significantly lower than that of the WT,along with excessive accumulation of reactive oxygen species(ROS).In the lmes7 mutant,the effective tiller number,plant height,flag leaf length,flag leaf width,panicle length,grain number per panicle,seed setting rate,and grain yield per plant decreased significantly,while grain thickness increased significantly.Genetic analysis indicated that the mutant phenotype was controlled by a single recessive nuclear gene.Using map-based cloning,the target gene was fine-mapped to a 90-kb region between RM28486 and RM28489 on chromosome 12.PCR and sequencing revealed a 7-bp deletion in OsACL-A2,a gene encoding ATP-citrate lyase,in the lmes7 mutant,resulting in a frameshift mutation and premature termination of translation.Phylogenetic analysis and sequence alignment indicated that the mutation site of lmes7 lies within the succinyl-CoA synthetase domain,identifying lmes7 as a novel allelic variant of OsACL-A2.关键词
水稻/类病变早衰/lmes7突变体/表型鉴定/精细定位Key words
rice/lesion mimic and early senescence/lmes7/phenotypic identification/fine mapping引用本文复制引用
刘亚萍,董译词,郑君妍,邱绚,刘鹏程,叶亚峰,刘斌美,陈析丰,马伯军..水稻类病变早衰突变体lmes7的鉴定与基因精细定位[J].中国水稻科学,2026,40(1):85-94,10.基金项目
国家自然科学基金资助项目(32071987,32101697) （32071987,32101697）
浙江省自然科学基金资助项目(LQ22C130005,LZ23C130004,Z24C130016). （LQ22C130005,LZ23C130004,Z24C130016）
