中国水稻科学2026，Vol.40Issue(1)：95-105,11.DOI:10.16819/j.1001-7216.2026.241110
水稻种子耐储性基因的挖掘及不同老化方式的转录组分析
Identification of Genes for Rice Seed Storability and Transcriptome Analysis Under Different Aging Conditions
摘要
Abstract
[Objective]Seed storability is crucial for germplasm conservation,food security,and sustainable development.This study aims to identify QTLs associated with rice seed storability and explore the genetic basis of seed storability under different aging conditions.[Method]A set of 131 Oryza nivara introgression lines in the genetic background of the indica variety 93-11 was used.Seeds were subjected to natural aging and artificial aging treatments,and germination rates were measured for QTL mapping.RNA sequencing was performed to analyze genome-wide gene expression patterns in embryos from the storage-tolerant line Ra32(carrying the O.nivara-derived allele of qASS1.1/qNSS1.1)and the storage-sensitive line Ra146(without the allele).GO and KEGG enrichment analyses were conducted to explore enriched pathways and to identify genes related to seed storability.[Result]Six QTLs for seed storability were identified on chromosomes 1,3,6,7,and 9,with individual QTLs explaining 3.3%to 21.0%of phenotypic variance.Among them,qASS1.1/qNSS1.1 was consistently detected under both natural and artificial aging treatments,and the O.nivara-derived allele at this QTL enhanced seed storability.Transcriptome analysis revealed 2077 differentially expressed genes(DEGs)under artificial aging and 1468 DEGs under natural aging,with 733 shared DEGs between the two treatments.GO and KEGG analyses showed that DEGs under artificial aging were enriched in 1428 GO terms and 97 metabolic pathways,while DEGs under natural aging were enriched in 1199 GO terms and 85 pathways.Two biological processes(circadian regulation of translation and hydrogen peroxide catabolic process)and 12 metabolic pathways(including MAPK signaling pathway,zeatin biosynthesis,histidine metabolism,and ascorbate and aldarate metabolism)were commonly enriched in both treatments,suggesting that seed storability may be regulated by antioxidant systems,energy metabolism,and accumulation of seed storage substances.Based on functional annotation of DEGs near the qASS1.1/qNSS1.1 locus,five candidate genes for qASS1.1/qNSS1.1 were further identified.[Conclusion]This study identified QTLs related to seed storability and screened multiple candidate genes,revealing that rice seeds enhance storability under different aging treatments mainly by regulating antioxidant defense,maintaining cellular stability,and modulating metabolic processes.These findings provide new genetic resources for improving seed storability in rice.关键词
野生稻/渗入系/QTL/耐储性/转录组/差异表达基因Key words
wild rice/introgression line/QTL/seed storability/transcriptome/differentially expressed gene引用本文复制引用
廖政明,郭梁,潘孝武,黎用朝,董铮,李小湘..水稻种子耐储性基因的挖掘及不同老化方式的转录组分析[J].中国水稻科学,2026,40(1):95-105,11.基金项目
国家水稻产业技术体系资助项目(CARS-01-19). （CARS-01-19）
