中国水稻科学2026，Vol.40Issue(1)：106-117,12.DOI:10.16819/j.1001-7216.2026.250112
粳稻花时性状QTL定位与聚合效应分析
QTL Mapping and Pyramiding Effect Analysis of Diurnal Floret Opening Time Traits in japonica Rice
摘要
Abstract
[Objective]Diurnal flowering time(FT)of rice is an important trait that affects the outcrossing rate and seed production yield of japonica rice sterile lines.This study aims to locate QTLs related to diurnal-floret-opening-time(DFOT)in japonica rice and analyze the pyramiding effects to clarify the main effect loci for DFOT traits.[Method]Recombinant inbred line populations consisting of 221 lines were constructed using japonica maintainer line J65B with early floret-opening-time and J139B with late floret-opening-time.The floret-opening-time was investigated in three years.QTL mapping analysis was conducted using genetic mapping and BSA-seq methods.[Result]A total of 30 DFOT QTLs were detected using complete interval mapping(ICIM)method on chromosomes 2-12.qFT5.2,qFT5.3,qFT5.5,qFT10.2,and qFT10.3 were major and stable QTLs which were detected simultaneously in more than eight environments,with an additive effect range of 13.04-50.93.Based on floret-opening time,extreme individuals were pooled for BSA-seq analysis.Association intervals related to floret-opening-time were detected in the regions of qFT5.3,qFT5.5,qFT6.2,and qFT10.2.According to the genotyping results,different progenies were divided into J139B type and J65B type in each QTL interval.QTL pyramiding effect analysis showed that QTLs derived from J65B would advance diurnal-floret-opening-time.The loci of qFT5.3 and qFT6.2 were essential for achieving early diurnal-floret-opening-time.The loci of qFT5.5 and qFT10.2 could further advance the diurnal-floret-opening-time.[Conclusion]Genetic mapping and BSA-seq analysis successfully identified four major stable QTLs related to rice diurnal-floret-opening-time.The localization of qFT5.3,qFT5.5,qFT6.2,and qFT10.2 establishes a foundation for further fine mapping and gene cloning and genetic improvement of diurnal-floret-opening-time in japonica rice.关键词
粳稻/花时/重组自交系/BSA-seq/QTL定位Key words
japonica rice/diurnal-floret-opening-time/recombinant inbred line/BSA-seq/QTL mapping引用本文复制引用
张城,孙廉平,邵国军,张雪,田书军,孙驰,郭艳颖,周燃,韩勇,郑文静..粳稻花时性状QTL定位与聚合效应分析[J].中国水稻科学,2026,40(1):106-117,12.基金项目
辽宁省自然科学基金面上项目"杂交粳稻保持系早花时QTL定位"(2022-MS-059) （2022-MS-059）
辽宁省农业科学院学科建设计划项目(2024XKJS5222). （2024XKJS5222）
