中国医科大学学报2026，Vol.55Issue(1)：58-63,6.DOI:10.12007/j.issn.0258-4646.2026.01.010
半夏提取物通过NLRP3/caspase-1通路对慢性阻塞性肺疾病大鼠肺损伤和气道炎症的影响
Effects of Pinellia ternata extract on lung injury and airway inflammation in rats with chronic obstructive pulmonary disease via NLRP3/caspase-1 pathway
摘要
Abstract
Objective To investigate the effect of Pinellia ternata extract on lung injury and airway inflammation in rats with chronic obstructive pulmonary disease(COPD)based on the nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3)/caspase-1 pathway.Methods Rats were randomly assigned into normal,COPD,low-dose Pinellia ternata extract,high-dose Pinellia ter-nata extract,and high-dose Pinellia ternata extract+nigericin groups(n=10 in each group).Lung function,inflammatory cell infiltration,and the levels of inflammatory cytokines in the bronchoalveolar lavage fluid(BALF)and serum were evaluated,and a pathological exam-ination of lung tissue was conducted.Western blotting was used to detect the expression of NLRP3/caspase-1 pathway-related proteins in lung tissue.Results In the COPD group(compared with the normal group)and the high-dose of Pinellia ternata extract+nigericin group(compared with the high-dose Pinellia ternata extract group),the lung capacity and peak expiratory flow(PEF)of rats were decreased,while the inspiratory resistance(Ri),lung pathological injury score,lymphocyte,neutrophil and eosinophil counts,levels of tumor necrosis factor-α(TNF-α),interleukin(IL)-1β,and IL-18,and relative expression of NLRP3 and caspase-1 proteins were increased(P<0.05).Compared with the COPD group,the lung capacity and PEF of rats in in low-and high-dose Pinellia ternata extract groups were increased,while Ri,the lung pathological injury score,lymphocyte,neutrophil,and eosinophil counts,levels of TNF-α,IL-1β,and IL-18,and relative expression of NLRP3 and caspase-1 proteins were decreased(P<0.05).The high-dose Pinellia ternata extract showed stronger improve-ment effects on various pathological indicators of rats with COPD.Conclusion Pinellia ternata extract can alleviate lung injury and airway inflammation in rats with COPD by inhibiting the activation of the NLRP3/caspase-1 pathway.关键词
NLRP3/caspase-1/半夏提取物/慢性阻塞性肺疾病/肺损伤/气道炎症Key words
NLRP3/caspase-1/Pinellia ternata extract/chronic obstructive pulmonary disease/lung injury/airway inflammation分类
医药卫生引用本文复制引用
陈旭亮,吴格怡,陈东明,关日辉,陈巧玲,黄心蔚..半夏提取物通过NLRP3/caspase-1通路对慢性阻塞性肺疾病大鼠肺损伤和气道炎症的影响[J].中国医科大学学报,2026,55(1):58-63,6.基金项目
广东省中医药局科研项目(20241298) （20241298）
湛江市科技计划(2022A01127) （2022A01127）
