安徽农业大学学报2025，Vol.52Issue(6)：985-993,9.DOI:10.13610/j.cnki.1672-352x.20260107.013
青海省当归根腐病菌鉴定及防治药剂筛选
Identification of root rot pathogens of Angelica sinensis from Qinghai Province and screening of chemical agents
摘要
Abstract
[Objective]This study aimed to clarify the species of root rot pathogens from Angelica sinensis in Qinghai Province,and screened effective fungicide for management this disease.[Method]Firstly,single spore iso-lation method was used to isolate the pathogens,and the pathogenicity of isolates was determined based on Koch's rule.Secondly,morphological characteristics of the pathogens were observed,and the sequences of rDNA-ITS and TEF-1α genes were amplified,sequenced,compared and combined to construct phylogenetic tree for molecular identification of pathogens.Finally,the inhibitory effects of seven fungicides on the hypha of pathogens were deter-mined by mycelial growth rate method for screening of effective fungicide.[Result]From 2020 to 2022,72 A.sinensis root rot samples with wilted aboveground parts and brown rotted roots were collected from Minhe,Huang-zhong,and Ledu counties in Qinghai Province,where disease incidence ranged from 15%to 45%.Totally,87 fungi isolates were isolated and all of them were pathogenic to the root of A.sinensis.The pathogens were Fusarium solani,F.avenaceum,F.acuminatum,F.flocciferum,F.equiseti and Clonostachys rosea according to identification results,and the dominant pathogens were F.solani,F.avenaceum and F.acuminatum with the isolation frequency of 33.4%,27.6%and 26.4%,respectively.Laboratory fungicide experiments demonstrated that flusilazole·prochloraz exhibited the strongest inhibition against the six pathogens,with EC50 value ranging from 0.011 to 1.051 mg·L-1.Difenocona-zole 50%SC demonstrated good efficacy against F.acuminatum,F.avenaceum,F.flocciferum,and F.solani(EC50 of 0.007-0.393 mg·L-1)but with poor inhibition rates against C.rosea and F.equiseti.Lime sulfur had the weakest inhibitory effect on all six pathogens,with EC50 values between 1.853 and 4.701 mg·L-1.[Conclusion]This study preliminarily identifies species of root rot pathogens from A.sinensis in Qinghai Province,and provides a scientific basis for rational selecting fungicides to control root rot of A.sinensis in production.关键词
当归/根腐病/镰刀菌/病原多样性/药剂筛选Key words
Angelica sinensis/root rot/Fusarium spp./diversity of pathogenic fungi/screening of fungicide分类
农业科技引用本文复制引用
祁鹤兴,李沐阳,段晓明..青海省当归根腐病菌鉴定及防治药剂筛选[J].安徽农业大学学报,2025,52(6):985-993,9.基金项目
浙江省重点研发计划项目(2023C04023) （2023C04023）
