中国耳鼻咽喉头颈外科2025，Vol.32Issue(11)：719-723,5.DOI:10.16066/j.1672-7002.2025.11.008
小鼠鼻腔细菌的评估、清扫及药敏研究
Evaluation and clearance of nasal bacteria in mice
摘要
Abstract
OBJECTIVE To evaluate sampling methods and clearance strategies for nasal bacteria in mice,providing reliable protocols for nasal microbiome research.METHODS Using nasal tissue(NT)bacterial culture counts as a reference,the suitability of pressing the nares(PN)and nasal lavage fluid(NLF)sampling methods for detecting nasal bacteria in mice was compared.Furthermore,NLF samples underwent bacterial culture and antibiotic susceptibility testing to assess the effectiveness of four clearance strategies:(1)free drinking of 5%glucose(GS)plus a four-antibiotic combination(4ABX),(2)free drinking of flavored water(FW)plus 4ABX,(3)free drinking of purified water,(4)intranasal instillation(i.n.)of a three-antibiotic combination(3ABX).RESULTS Compared with NLF and NT methods,the PN method yielded significantly higher numbers of Escherichia coli(P=0.041).No significant differences in bacterial profiles were observed between NT and NLF samples.Both the 3ABX i.n.group and the 5%GS+4ABX group achieved significantly higher nasal bacteria clearance rates than the FW+4ABX group(P<0.05).Primary residual colonies were antibiotic-insensitive.CONCLUSION NLF and NT sampling methods yield highly consistent bacterial profiles,making both suitable for mouse nasal microbiome studies.Local and systemic antibiotic treatments effectively reduce nasal bacterial loads;however,the persistence of resistant strains underscores the need for optimized clearance strategies.关键词
小鼠/鼻腔/滴注,药物/培养技术/滴鼻/抗生素/细菌培养Key words
Mice/Nasal Cavity/Instillation,Drug/Culture Techniques/intranasal/antibiotic/bacterial culture引用本文复制引用
闫冰,任益民,刘向祎,黄艳飞,张桂,王成硕,张罗..小鼠鼻腔细菌的评估、清扫及药敏研究[J].中国耳鼻咽喉头颈外科,2025,32(11):719-723,5.基金项目
国家自然科学基金(82025010、82171108) （82025010、82171108）
北京市卫生系统高层次公共卫生人才建设项目(学科骨干-03-03) （学科骨干-03-03）
北京市卫生健康委员会市属医学科研院所公益发展改革试点项目(JYY2023-1) （JYY2023-1）
