陆军军医大学学报2026，Vol.48Issue(2)：107-115,9.DOI:10.16016/j.2097-0927.202512008
CD4+T细胞通过分泌IFN-γ促进伯氏疟原虫红内期感染虫荷的机制研究
CD4+T cells promote Plasmodium berghei blood-stage burden via IFN-γ secretion
摘要
Abstract
Objective To investigate the role and mechanism of CD4+T-cell-derived IFN-γ in host defense against blood-stage Plasmodium berghei(P.berghei)infection.Methods A tamoxifen-inducible conditional knockout strategy was performed to generate mice lacking IFN-γ specifically in CD4+T cells(Ifngfl/flCD4-CreERT2).Then after the mice were infected intravenously with luciferase-expressing P.berghei ANKA(P.berghei ANKA-luciferase)strain,parasite burden,survival,and intra-erythrocytic developmental rhythm were monitored dynamically.Flow cytometry and ELISA were carried out respectively to quantify the splenic myeloid subsets and the titers of P.berghei-specific IgG and IgG2c.Results CD4+T-cell-specific IFN-γ deficiency significantly reduced peripheral parasitemia on day 5 post-infection(P<0.05),showing no effect on survival or 24-hour intra-erythrocytic cycle of the parasite.Ifngfl/fl CD4-CreERT2 mice exhibited obviously decreased P.berghei-specific IgG2c(P<0.01)but increased total IgG(P<0.05).Frequencies and absolute numbers of neutrophils,monocytes,and macrophages remained unchanged.Conclusion During the process of P.berghei infection,IFN-γ secreted by CD4+T cells may decrease total serum IgG level by promoting class switching to IgG2c,and then enhance parasite burden after blood-stage P.berghei infection.关键词
CD4+T细胞/干扰素-γ/伯氏疟原虫/体液免疫Key words
CD4+T cells/IFN-γ/Plasmodium berghei/humoral immunity分类
医药卫生引用本文复制引用
李航宇,高源利,范永铃,徐文岳,刘太平..CD4+T细胞通过分泌IFN-γ促进伯氏疟原虫红内期感染虫荷的机制研究[J].陆军军医大学学报,2026,48(2):107-115,9.基金项目
Supported by the Gereral Program of National Natural Science Foundation of China(82172296).国家自然科学基金面上项目(82172296) （82172296）
