法医学杂志2025，Vol.41Issue(5)：456-467,12.DOI:10.12116/j.issn.1004-5619.2025.450404
温度对尸体不同组织微生物群落演替及死亡时间推断的影响
Effect of Temperature on Microbial Succession in Different Tissues of Cadavers and Estimation of Postmortem Interval
摘要
Abstract
Objective To explore the distribution characteristics of microbial communities in various rat tissues under different temperature conditions and their dynamic changes over the postmortem interval(PMI),and to analyze the effects of temperature and tissue type on microbial succession in cadavers.Methods A total of 96 rats were sacrificed by cervical dislocation and then placed under room tem-perature(20℃,n=48),high temperature(40℃,n=24),and low temperature(-20℃,n=24)condi-tions.Tissue samples from the diaphragm,lung,rectum,testis,and uterus were collected at various PMIs.Microbial community composition was analyzed using 16S rRNA high-throughput sequencing of the 16S rRNA gene V3-V4 regions.α-diversity,β-diversity,phylum-and genus-level species distribu-tions,PMI-associated biomarkers analysis and species differential analysis were employed to systemati-cally compare the effects of temperature and tissue type on microbial succession.Results Under room temperature,microbial diversity exhibited a nonlinear trend,initially increasing and then decreasing.High temperature condition accelerated microbial succession and resulted in a significant decrease in microbial diversity within 24 hours.Low temperature slowed the succession,maintaining relatively high diversity and stable species distribution.The rectal microbial community differed significantly from those in other tissues.The phylum Proteobacteria,especially the genus Proteus,showed a signifi-cant increase in relative abundance in various tissues after 48 hours at room temperature and 24 hours at high temperature.Conclusion The dynamic succession patterns of microbial communities in multiple tissues under different temperature conditions confirm the significant regulatory effect of temperature on microbial diversity and species distribution,providing an important basis for optimizing microbiome-based PMI estimation methods.关键词
法医病理学/法医微生物学/微生物组学/死亡时间推断/16S rRNA/温度/大鼠Key words
forensic pathology/forensic microbiology/microbiomics/postmortem interval estimation/16S rRNA/temperature/rats分类
医药卫生引用本文复制引用
陈吉,张素华,赵俞蓉,黄馨,屈轶龄,路艳芳,邢宇,张晗,曾建业,李士林..温度对尸体不同组织微生物群落演替及死亡时间推断的影响[J].法医学杂志,2025,41(5):456-467,12.基金项目
国家自然科学基金资助项目(82371896,82471920) （82371896,82471920）
