法医学杂志2025，Vol.41Issue(5)：477-481,5.DOI:10.12116/j.issn.1004-5619.2025.450301
qPCR检测尸体心血浮游生物DNA诊断溺死
Diagnosis of Drowning by qPCR Detection of Plankton DNA in Cardiac Blood of Cadavers
摘要
Abstract
Objective To investigate the feasibility of diagnosing drowning by detecting plankton DNA in cardiac blood of cadavers recovered from water using real-time quantitative PCR(qPCR).Methods Cardiac blood samples were collected via precordial puncture from 32 drowned cadavers and 10 non-drowned cadavers.qPCR was employed to detect plankton(diatoms,cyanobacteria and Aeromonas)in the blood samples.Results Plankton DNA was detected in 17 drowning cadavers,while no plankton DNA was detected in the 10 non-drowned cadavers.Conclusion qPCR can detect plankton DNA in cardiac blood of most drowning cases.This method may have potential as an auxiliary tool in the diag-nosis of drowning.关键词
法医病理学/溺死/实时荧光定量PCR/心血/浮游生物Key words
forensic pathology/drowning/real-time quantitative PCR/cardiac blood/plankton分类
医药卫生引用本文复制引用
张晓峰,杨幸怡,徐曲毅,陈玲,陈晓晖,赵建,林添春,谷党恩,王锋,刘志勇,蒙诗韵..qPCR检测尸体心血浮游生物DNA诊断溺死[J].法医学杂志,2025,41(5):477-481,5.基金项目
国家自然科学基金资助项目(82371901) （82371901）
国家重点研发计划资助项目(2023YFC3303902) （2023YFC3303902）
公安部科技计划资助项目(2022JC35) （2022JC35）
公安部刑事技术"双十计划"推广项目(2022SSTG01) （2022SSTG01）
