法医学杂志2025，Vol.41Issue(5)：494-501,8.DOI:10.12116/j.issn.1004-5619.2024.440115
铁死亡在小鼠百草枯急性肺损伤中的作用
Role of Ferroptosis in Paraquat-Induced Acute Lung Injury in Mice
摘要
Abstract
Objective To explore the potential mechanisms by which ferroptosis contributes to paraquat-induced acute lung injury in mice and its forensic significance.Methods Sixty healthy male clean-grade Kunming mice aged 6-8 weeks were randomly divided into a control group and paraquat expo-sure groups(1 d,3 d,5 d,7 d and 14 d),with 10 mice per group.The exposure groups were given a single intraperitoneal injection of 20 mg/kg paraquat solution,while the control group was given an equal volume of 0.9%saline.General conditions after exposure were observed.Hematoxylin-eosin(HE)staining was used to examine pathological changes in lung tissues,and Prussian blue staining was used to detect iron content.Oxidative stress indicators,such as reduced glutathione(GSH)and malondialdehyde(MDA),were measured by relevant testing kits.Immunohistochemistry and Western blot were employed to detect the expression levels of ferroptosis-related proteins including glutathione peroxidase 4(GPX4),solute carrier family 7 member 11(SLC7A11),and heme oxygenase-1(HO-1).Results With the increase of exposure time,mice in the exposure groups showed progressively aggra-vated fibrosis and increased iron deposition in lung tissues.GSH levels gradually decreased,while MDA levels increased significantly in the 7 d and 14 d groups.The expression of GPX4 in lung tissue decreased.The expression of SLC7A11 decreased over time,except for the 1 d group.The expression of HO-1 increased with the increase of exposure time,except for the 1 d group,and the highest ex-pression was observed in the 7 d group.The differences between the exposure groups and the control group were statistically significant(P<0.05).Conclusion Ferroptosis is involved in acute lung injury induced by paraquat poisoning,and research on the mechanism of ferroptosis and the temporal changes of its biomarkers may provide valuable insights for the forensic identification of paraquat poisoning.关键词
法医病理学/法医毒理学/百草枯/急性肺损伤/细胞铁死亡/小鼠Key words
forensic pathology/forensic toxicology/paraquat/acute lung injury/ferroptosis/mice分类
医药卫生引用本文复制引用
秦豪杰,莫晨,李洪伟,刘之江,郑哲..铁死亡在小鼠百草枯急性肺损伤中的作用[J].法医学杂志,2025,41(5):494-501,8.基金项目
河南省科技攻关计划项目(232102310352) （232102310352）
