医学分子生物学杂志2026，Vol.23Issue(1)：27-35,9.DOI:10.3870/j.issn.1672-8009.2026.01.004
海藻酸盐-银-S-亚硝基谷胱甘肽纳米颗粒凝胶治疗幽门螺旋杆菌感染小鼠模型的抗菌活性研究
Antibacterial Activity of Alginate-Silver-S-Nitrosoglutathione Nanop-article Gel in Treatment of Helicobacter Pylori Infected Mouse Model
摘要
Abstract
Objective To investigate the antibacterial activity of alginate-silver-S-nitrosogluta-thione nanoparticle(NP)gel in the treatment of Helicobacter pylori(H.pylori)infected mouse model.Methods Alginate NPs gel,alginate-Ag NPs gel and alginate-Ag-GSNO NPs gel were prepared.The surface characteristics of Ag-GSNO nanoparticle gel were imaged by scanning electron microscopy.A mouse model of H.pylori infection was established.A total of 40 male C57BL/6 mice were randomly divided into 4 groups with 10 mice each:Vehicle group,Model group,Alginate-Ag NPs gel group,Alginate-AG-GSNO NPs gel group.The colony forming unit(CFU)counting of H.pylori infection in mice gastric epithelium was performed with blood agar culture.HE staining and histopathology were used to analyze the inflammation and injury of gastric epithelium in mice.The expression levels of ureB gene,which is a positive marker of H.pylori infection,was determined by qPCR.The levels of inflammatory cytokines TNF-α,IL-1β and IL-6 were determined by ELISA.The expression levels of NLRP3 inflammasome was determined by Westren blotting.Results Alginate-Ag nanoparticles had a spherical morphology,with a particle size range of 100-200 nm.Spray dried GSNO particles were spherical with slight depressions,and their size was at the μm level.Compared with those in the Alginate-Ag NPs gel group,the CFU of H.pylori infected mice gastric epithelial tissues was decreased(P<0.05),and the HE staining score was reduced(P<0.05)in the Alginate-AG-GSNO NPs gel group.The Gastric epithelial cells in the Alginate-AG-GS-NO NPs gel group were arranged orderly in the Alginate-AG-GSNO NPs gel group,and there were a reduced number of polymorphonuclear infiltration,lymphoid aggregation and H.pylori infection posi-tive cells,indicating the improvement of inflammation and injury of gastric epithelial tissues.The expression levels of ureB gene,the relative expression levels of NLRP3 inflammasome,and the lev-els of inflammatory cytokines TNF-α,IL-1β and IL-6 were all decreased(all P<0.05).Conclu-sion Compared with alginate-Ag NPs gel,alginate-AG-GSNO NPs gel could more effectively in-hibit the colonization of H.pylori in infected mouse model and improve gastric epithelium damage.关键词
幽门螺旋杆菌/海藻酸盐-银纳米颗粒凝胶/海藻酸盐-银-S-亚硝基谷胱甘肽纳米颗粒凝胶/胃上皮组织/抗菌活性Key words
Helicobacter pylori/alginate-Ag nanoparticles gel/alginate-Ag-GSNO nanoparti-cles gel/gastric epithelial tissue/antibacterial activity分类
医药卫生引用本文复制引用
卡斯木·卡哈尔,阿依努尔·吐鲁洪,阿迪力·阿不都热合曼..海藻酸盐-银-S-亚硝基谷胱甘肽纳米颗粒凝胶治疗幽门螺旋杆菌感染小鼠模型的抗菌活性研究[J].医学分子生物学杂志,2026,23(1):27-35,9.基金项目
新疆维吾尔族自治区自然科学基金(No.2023D01C329) This work was supported by a grant from the Natural Science Foundation of Xinjiang Uygur Autonomous Region(No.2023D01C329) （No.2023D01C329）
