医学分子生物学杂志2026，Vol.23Issue(1)：36-42,7.DOI:10.3870/j.issn.1672-8009.2026.01.005
lncRNA CRNDE通过ERK1/2通路影响溃疡性结肠炎小鼠体内巨噬细胞焦亡和M1型极化
Effect of lncRNA CRNDE on Macrophage Pyroptosis and M1-type Polarization in Ulcerative Colitis Mice via ERK1/2 Pathway
摘要
Abstract
Objective To investigate the effect of long non-coding RNA(lncRNA)CRNDE on macrophage pyroptosis and M1 polarization in ulcerative colitis(UC)mice and the potential mechanism.Methods The UC mouse model was established,and colon length,disease activity index(DAI)were measured to validate the model.RAW264.7 cells were divided into 4 groups:Ctrl group,pcDNA-null group,pcDNA-CRNDE group,and pcDNA-CRNDE+LY3214996 group.The expression levels of lncRNA CRNDE,cleaved-Caspase-1,cleaved-GSDMD,ERK1/2,and phosphorylated ERK1/2(p-ERK1/2)were detected by qRT-PCR or Western blotting.The proportion of F4/80+CD86+macrophages was measured by flow cytometry.Results In the model group,the DAI score was increased,the colon length was shortened,and the expression levels of lncRNA CRNDE,cleaved-Caspase-1,cleaved-GSDMD,and p-ERK1/2 were upregulated,and the proportion of F4/80+CD86+macrophages was increased(all P<0.05).In RAW264.7 cells,lncRNA CRNDE and cleaved-Caspase-1、cleaved-GSDMD、p-ERK1/2 expression in the pcDNA-CRNDE group was upregulated when compared with that in the pcDNA-null group,and that was downregulated after LY3214996 treatment(all P<0.05).Conclusion lncRNA CRNDE promotes macrophage pyroptosis and M1 polarization in UC mice by activating the ERK1/2 pathway,sugges-ting it may be a potential therapeutic target for UC.关键词
溃疡性结肠炎/lncRNA CRNDE/巨噬细胞/焦亡/M1 型极化Key words
ulcerative colitis/lncRNA CRNDE/macrophage/pyroptosis/M1 polarization分类
医药卫生引用本文复制引用
翡罗热·地里夏提,祖丽胡玛尔·阿力木江,杜进璇..lncRNA CRNDE通过ERK1/2通路影响溃疡性结肠炎小鼠体内巨噬细胞焦亡和M1型极化[J].医学分子生物学杂志,2026,23(1):36-42,7.基金项目
新疆维吾尔自治区自然科学基金(No.2023D01C423) This work was supported by a grant from the Natural Science Foundation of Xinjiang Uygur Autonomous Region(No.2023D01C423) （No.2023D01C423）
