医学分子生物学杂志2026，Vol.23Issue(1)：67-73,7.DOI:10.3870/j.issn.1672-8009.2026.01.009
NOP14过表达抑制NRIP1表达及卵巢癌SKOV3细胞的增殖与迁移
NOP14 Overexpression Inhibits NRIP1 Expression and Proliferation and Migration of Ovarian Cancer SKOV3 Cells
摘要
Abstract
Objective To investigate the effect of nucleolar protein 14(NOP14)overexpressi on proliferation and migration of ovarian cancer cells SKOV3 and the underlying mecha-nism.Methods The expression level of NOP14 in ovarian cancer cell lines(SKOV3,A2780,HO-8910,OVCAR)was detected by RT-qPCR and Western blotting.SKOV3 cells were transfected with the pcDNA-NOP14 plasmid.Cell proliferation was detected by colony formation assay and 5-E-thynyl-2′-deoxyuridine(EdU)staining.Tumor stem cell characteristics were analyzed via serum-free sphere formation assay.The proportion of CD133-positive(CD133+)cells was measured by flow cytometry.The epithelial-mesenchymal transition(EMT)process was evaluated by observing cell morphological change and detecting the EMT marker proteins(E-cadherin,N-cadherin,Vim-entin).The expression level of nuclear receptor interacting protein 1(NRIP1),and Wnt/β-catenin pathway proteins were detected by RT-qPCR and Western blotting.Results The expression level of NOP14 was significantly lower in the ovarian cancer cell lines than that in the normal ovarian epithe-lial cells.Overexpression of NOP14 significantly inhibited the proliferation,stem cell characteris-tics,and EMT of SKOV3 cells(mesenchymal markers N-cadherin and Vimentin were downregulat-ed,while the epithelial marker E-cadherin was upregulated).Moreover,Overexpression of NOP14 suppressed the expression of NRIP1,Wnt and β-catenin.Conclusion NOP14 can inhibit the pro-liferation,stem cell characteristics,and EMT process of ovarian cancer SKOV3 cells,and nega-tively regulate the expression of NRIP1,Wnt,β-catenin.关键词
核仁蛋白14/卵巢癌/核受体相互作用蛋白1/细胞增殖/上皮-间质转化Key words
nucleolar protein 14/ovarian cancer/nuclear receptor interacting protein 1/cell proliferation/epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
李苓妙,李萍,胡晓丽,王冬梅,武子先..NOP14过表达抑制NRIP1表达及卵巢癌SKOV3细胞的增殖与迁移[J].医学分子生物学杂志,2026,23(1):67-73,7.基金项目
河北省2022年度医学科学研究课题(No.20221906) This work was supported by a grant from the Hebei Province 2022 Annual Medical Science Research Topic(No.20221906) （No.20221906）
