贵州农业科学2026，Vol.54Issue(1)：1-12,12.DOI:10.3969/j.issn.1001-3601.2026.01.001
水稻ABF转录因子家族的全基因组鉴定及表达模式分析
Genome-wide Identification and Expression Pattern Analysis of Rice ABF Transcription Factor Family
摘要
Abstract
[Objective]The physicochemical properties,structure characteristics and expression pattern of OsABF gene family members in rice were explored and verified to lay a foundation for biological functions of rice OsABF gene family.[Method]The members of rice OsABF family were identified by bioinformatics,and their phylogenetic relationships,gene structure,conserved motifs and collinearity,promoter elements and expression patterns in different tissues were analyzed.The expression patterns of OsABF genes under different abiotic stresses were analyzed by qRT-PCR technology.[Result]11 OsABF genes were identified from rice genome and named as OsABF1-OsABF11.The protein sequence length,size and isoelectric point of OsABF1-OsABF11 were 244-388 amino acids with an average length of 332 amino acids,25.48-41.48 kDa with an average of 35.40 kDa and 5.30-9.56,respectively.11 OsABF protein were divided into two subfamilies of A(OsABF4,OsABF7,OsABF8,OsABF10 and OsABF11)and B(OsABF1,OsABF2,OsABF3,OsABF5,OsABF6 and OsABF9).OsABF was relatively conserved in two subfamilies.The segmental duplication was the main factor driving evolution of rice OsABF gene family and the purifying selection played an important role in the evolution of rice OsABF gene family.The response of 8 plant hormones,regulation of 6 plant growing development and 5 cis-acting elements under abiotic and biological stresses were respectively identified from upstream promoters of OsABF genes,and the sequence of all OsABF promoters had ABRE cis-acting elements.The expression profile analysis showed that expression of most OsABF genes in subfamily A was in different tissues and induced by abiotic stresses such as drought,low temperature,ABA,and JA.[Conclusion]11 OsABF protein are divided into A and B subfamily with a relative conserved function.OsABF4 and OsABF10 in A subfamily play an important role in rice growing development and the response to abiotic stress.关键词
水稻/ABF转录因子家族/全基因组鉴定/表达模式分析/生物信息学Key words
rice/ABF transcription factor family/genome-wide identification/expression pattern analysis/bioinformatics分类
农业科技引用本文复制引用
安畅,龙江辉,黄培英,徐灵,李鲁华..水稻ABF转录因子家族的全基因组鉴定及表达模式分析[J].贵州农业科学,2026,54(1):1-12,12.基金项目
贵州省农业科学院喀斯特山区作物基因资源与种质创新重点实验室项目"优质高产水稻材料创新与品种选育"(黔农科科技创新[2023]06号) （黔农科科技创新[2023]06号）
贵州省科技成果转化项目"优质高产杂交稻香两优贵福占的应用与示范(黔科合成果[2024]一般068) （黔科合成果[2024]一般068）
