贵州农业科学2026，Vol.54Issue(1)：38-47,10.DOI:10.3969/j.issn.1001-3601.2026.01.005
过量铵态氮水培处理对烟草幼苗叶片转录组的影响
Impact of Excess Ammonium Nitrogen Treatment on Leaf Transcriptome of Tobacco Seedlings Under Hydroponic Culture
摘要
Abstract
[Objective]The effects of excessive ammonium nitrogen on leaf gene expression and metabolic pathways of tobacco seedlings were investigated to enrich the molecular mechanisms of plants in response to high ammonium stress and provide a scientific basis for rational application of ammonium fertilizer in agricultural production.[Method]The phenotypic difference in the above-ground part of tobacco seedlings between CK(0.5 mmol NH4+)and excessive ammonium treatment(20.0 mmol NH4+)was compared by using a hydroponic system and the leaf transcriptome of two treatments was analyzed.The part key differentially expressed genes(DEGs)in leaves of tobacco seedlings from the excessive ammonium treatment and CK were detected by using RT-qPCR.[Result]The excessive ammonium treatment significantly inhibited growth of tobacco seedlings and caused curling leaves with a dark green color,and wilting and yellowing symptoms.A total of 3 183 DEGs were screened from leaves of tobacco seedlings of the excessive ammonium treatment and CK,and the up-regulated and down-regulated genes accounted for 66.04%and 33.96%respectively.The GO and KEGG enrichment analyses showed that the DEGs were mainly enriched in pathways of photosynthesis-light energy capture protein,phenylpropane biosynthesis,starch and sucrose metabolism and carbon fixation in photosynthesis.The RT-qPCR analysis showed that the expression change of part leaf key DEGs of tobacco seedlings treated with excessive ammonium nitrogen was consistent with the transcriptome sequencing results.[Conclusion]The excessive ammonium nitrogen inhibits growth of tobacco seedlings by affecting the expression of genes involved in key relative pathways,such as the photosynthetic light reaction and carbohydrate metabolism.关键词
烟草/铵态氮/转录组/差异表达基因/代谢途径/富集分析Key words
tobacco/ammonium nitrogen/transcriptome/differentially expressed gene/metabolic pathway/enrichment analysis分类
农业科技引用本文复制引用
石楚含,王倩,苏晓晴,崔萌萌,徐刚,李世金,王以慧,侯冰清,宁扬,毛东平..过量铵态氮水培处理对烟草幼苗叶片转录组的影响[J].贵州农业科学,2026,54(1):38-47,10.基金项目
安徽省烟草公司科技项目"皖南烟区低山丘陵坡地烟叶栽培关键技术研究与应用"(2023341800040464) （2023341800040464）
山东中烟工业有限责任公司重大专项"茄衣烟叶生产关键技术集成与应用"(2022370000340625) （2022370000340625）
