贵州农业科学2026，Vol.54Issue(1)：77-86,10.DOI:10.3969/j.issn.1001-3601.2026.01.009
火龙果蔗糖转化酶基因(HuINVs)的全基因组鉴定与分析
Genome-wide Identification and Analysis of Sucrose Invertase Gene(HuINVs)in Pitaya
摘要
Abstract
[Objective]The biological characteristics and potential function of sucrose invertases(HuINVs)encoded by pitaya whole genome and their corresponding gene(HuINVs)family were explored to provide a theoretical basis for improving quality of pitaya.[Method]The HuINVs encoded by pitaya whole genome and their corresponding HuINVs genes were screened from red flesh pitaya and yellow pitaya by bioinformatics analysis.The evolutionary relationships,physicochemical properties,sequence features and expression patterns of these HuINVs were systematically analyzed.[Result]A total of 22 HuINVs including 12 cytoplasm invertases(HuCINs),6 cell wall invertases(HuVINs)and 4 vacuolar invertases(HuCWINs)were identified from the proteome of red flesh pitaya.There were minimal differences in physicochemical property and sequence characteristics between HuINVs of the same type.The genetic relationship of sucrose invertase protein between red flesh pitaya and yellow pitaya was closer.There were multiple cis-acting elements in the upstream promoter region of initiation codons of HuINVs.Except for the most common photoresponse cis-acting elements,most cis-acting elements were related to abscisic acid and some cis-acting elements were related to stress response and growing development.There was a difference in expression of HuINVs genes in different tissues.The expression quantity of HuVIN1,HuCIN5 and HuCWIN3 in flowers was higher.The expression quantity of HuINVs genes in fruits was higher than in flower buds and flowers.The expression quantity of HuCIN5 gene reached the highest on the 65th day of fruit development.[Conclusion]The HuINVs of the same type are highly conserved in sequence,and the HuINVs gene expression is of tissue specificity and stage specificity in pitaya.关键词
火龙果/燕窝果/蔗糖转化酶/全基因组/家族分析/表达分析/生物信息学Key words
pitaya/yellow pitaya/sucrose invertase/whole genome/family analysis/expression analysis/bioinfor-matics分类
农业科技引用本文复制引用
王荔,王恩权,王宇,方晓彤,张雪..火龙果蔗糖转化酶基因(HuINVs)的全基因组鉴定与分析[J].贵州农业科学,2026,54(1):77-86,10.基金项目
贵州省科技支撑计划项目"喀斯特地区山地果园生产保障修复及生态增效技术示范研究"(黔科合支撑[2023]一般248),"利用冠突散囊菌延长水果货架期的技术研究"(黔科合支撑[2023]一般085) （黔科合支撑[2023]一般248）
贵州省农业科学院喀斯特山区作物基因资源与种质创新重点实验室项目"调控火龙果种子大小的关键基因挖掘研究"(黔农科一般基金[2025]50) （黔农科一般基金[2025]50）
黔南州科技计划项目"罗甸老龄火龙果园提质增效改造关键技术研究与示范"(黔南科合[2024]105号) （黔南科合[2024]105号）
