亚热带农业研究2025，Vol.21Issue(4)：280-288,9.DOI:10.13321/j.cnki.subtrop.agric.res.2025.04.008
库拉索芦荟黑斑病病原鉴定及防控药剂筛选
Pathogen identification of Aloe barbadensis black spot disease and screening of fungicides
摘要
Abstract
[Purpose]This study aims to identify the pathogen causing the black spot disease of Aloe barbadensis in Fujian Province and explore the biological characteristics of the pathogen,thus providing a theoretical basis for the control of this disease.[Method]The black spot disease samples of A.barbadensis were collected from Putian City,Fujian Province,China.The pathogenic strains were isolated and purified by the tissue isolation method,and the pathogenicity of the strains was tested according to Koch's rule.The inoculated strains causing the disease again were identified based on multiple gene sequences.The toxicity of fungicides against the pathogens was determined based on the inhibition rates against mycelial growth.[Result]A total of 12 strains were isolated from the diseased leaves of A.barbadensis,and 2 pathogenic strains were ultimately screened out via the re-inoculation method and named as PT3 and PT11.On the basis of morphological observations and multi-gene phylogenetic analysis,strain PT3 was identified as an unknown species of Alternaria,and strain PT11 showed a similarity of over 99%to Aureobasidium.PT3 and PT11 optimal pH is 6-7;the optimum temperature is 20-25℃.Alternating light and dark for 12 h was the most suitable for the growth of strain PT3,while strain PT11 was not sensitive to the light cycle.The optimal carbon and nitrogen sources for both strains were glucose and pep-tone,respectively.96%iprodione wettable powder(50 μg•mL-1)had the best inhibitory effect on strain PT3,with a median ef-fective concentration(EC50)of 1.120 μg•mL-1.98%carbendazim wettable powder(1.00 μg•mL-1)showed the strongest inhibi-tory effect on strain PT11,with an EC50 of 0.152 μg•mL-1.[Conclusion]Two pathogenic fungal strains were isolated from the black spot disease samples of A.barbadensis,with PT3 belonging to Alternaria and PT11 belonging to Aureobasidium.The optimal growth conditions for both strains are 20-25℃,pH 6-7,glucose as the carbon source,and peptone as the nitrogen source.The best fungicides for PT3 and PT11 are 96%iprodione wettable powder and 98%carbendazim wettable powder,respectively.关键词
库拉索芦荟/黑斑病/病原菌鉴定/生物学特性/杀菌剂筛选Key words
Aloe barbadensis/black spot disease/identification of pathogen/biological characteristics/fungicide screening分类
农业科技引用本文复制引用
凌宇,谢雅文,王朝露,李正康,樊佶,陈晓婷..库拉索芦荟黑斑病病原鉴定及防控药剂筛选[J].亚热带农业研究,2025,21(4):280-288,9.基金项目
福建省科特派项目(2022-2024). （2022-2024）
