化工学报2025，Vol.76Issue(12)：6551-6561,11.DOI:10.11949/0438-1157.20250345
LysR家族转录调控因子对丁烯基多杀菌素生物合成的影响
Effect of LysR family transcriptional regulatory factors on the biosynthesis of butenyl-spinosyn
摘要
Abstract
Butenyl spinosyns are new green biopesticides produced by Saccharopolyspora pogona.However,due to their complex metabolic network,unclear genetic background and inadequately analyzed regulatory mechanisms,their synthesis efficiency is low and it is difficult to meet actual needs.In this study,comparative transcriptomics analysis of S.pogona ASAGF58(wild-type)and its high-yielding mutant ASAGF40 led to the identification of LysR family transcriptional regulators associated with secondary metabolism and butenyl-spinosyn biosynthesis.Engineered strains were subsequently constructed to validate their regulatory roles.Among them,RS 18275 significantly enhanced butenyl-spinosyn production to 67.1 mg/L,achieving a yield 2.3-fold higher than the parental strain ASAGF58,accompanied by a 30.63％enhancement in biomass and accelerated glucose consumption.Comparative transcriptomic analysis demonstrated that RS18275 promotes precursor supply(e.g.,acetyl-CoA)by regulating primary metabolic pathways and increases product biosynthetic flux by upregulating the expression of key genes involved in the butenyl-spinosyn biosynthetic pathway.Based on comparative transcriptomics analysis and verification,the physiological mechanism of RS18275 as a global regulatory factor to achieve efficient synthesis of target compounds by regulating primary metabolism and secondary metabolism was preliminarily analyzed,providing theoretical and engineering strategies for the subsequent efficient synthesis of butenyl spinosad.关键词
丁烯基多杀菌素/须糖多孢菌/转录调控因子/比较转录组学Key words
butenyl-spinosyn/Saccharopolyspora pogona/transcriptional regulatory factors/comparative transcriptomics分类
生物科学引用本文复制引用
陈霞,郭超,李新颖,王超,李春..LysR家族转录调控因子对丁烯基多杀菌素生物合成的影响[J].化工学报,2025,76(12):6551-6561,11.基金项目
十四五重点研发计划项目(2023YFA0914700) （2023YFA0914700）
工业生物催化教育部重点实验室(清华大学)开放基金资助项目(2023003,2024003) （清华大学）
