河南农业大学学报2026，Vol.60Issue(1)：64-76,13.DOI:10.16445/j.cnki.1000-2340.20250617.001
拟杆菌早期干预对大肠杆菌攻毒雏鸡肠道健康的影响
Effects of early intervention with Bacteroides on gut health in chickens challenged with E.coli
摘要
Abstract
[Objective]This study aimed to investigate the alleviating effects of early intervention with Bacteroides on intestinal health in chickens challenged with Escherichia coli(E.coli),providing new insights for sustainable and healthy poultry farming.[Method]A total of 150 newly hatched healthy arbor acres(AA)male chicks were randomly divided into three groups:(1)Control group(gavaged daily with 0.5 mL physiological saline from day 1 to day 8);(2)E.coli group(gavaged daily with 0.5 mL physiological saline from day 1 to day 7,followed by a challenge with 0.5 mL avian pathogenic E.coli suspension on day 8);and(3)Bacteroides group(gavaged daily with 0.5 mL Bacteroides sus-pension from day 1 to day 7,followed by a challenge with 0.5 mL avian pathogenic E.coli suspension on day 8).At 3 days(10 days of age)and 14 days(21 days of age)post-challenge,8 chickens per group were randomly selected for slaughter and sampling.Growth performance,serum biochemical and antioxidant indices,serum inflammatory cytokines,intestinal morphology,expression of intestinal barrier-related genes,and gut microbiota composition were analyzed.[Result]Early intervention with Bacteroides showed no significant effects on growth performance or immune organ indices in E.coli challenged chickens.However,it significantly enhanced total antioxidant capacity and serum supero-xide dismutase activity,improved serum inflammatory cytokine levels.The relative mRNA expression levels of OCLN,CLDN3,ZO1,and ZO2 in the jejunum,as well as CLDN3,ZO1,and ZO2 in the ileum,were significantly upregulated on day 3 post-challenge.On day 14 post-challenge,the relative mRNA expression levels of OCLN,CLDN3,ZO1,and ZO2 in both the jejunum and ileum were sig-nificantly increased.Additionally,Bacteroides intervention altered cecal microbiota composition,increasing the relative abundance of Bacteroides and reducing Escherichia_Shigella abundance.[Conclusion]Early intervention with Bacteroides significantly improved immune regulation in E.coli challenged chickens and reshaped gut microbiota colonization patterns,effectively alleviating pathogen-induced physiological damage.关键词
拟杆菌/禽致病性大肠杆菌/雏鸡/肠道健康/免疫调节Key words
Bacteroides/avian pathogenic E.coli/chickens/intestine health/immunoregulation分类
农业科技引用本文复制引用
侯萌,李嘉彤,慎思,曹忠正,蒋瑞瑞,康相涛,田亚东,宫玉杰..拟杆菌早期干预对大肠杆菌攻毒雏鸡肠道健康的影响[J].河南农业大学学报,2026,60(1):64-76,13.基金项目
河南省蛋鸡产业技术体系项目(HARS-22-18-S) （HARS-22-18-S）
河南省重点研发专项(241111113600) （241111113600）
河南农业大学科技创新基金科技协同创新专项(2024CXZX009) （2024CXZX009）
