河南农业大学学报2026，Vol.60Issue(1)：77-86,10.DOI:10.16445/j.cnki.1000-2340.20250428.003
猪circTEX9生物学特性及其对猪肌内前体脂肪细胞的影响
Biological characteristics of porcine circTEX9 and its effect on porcine intramuscular preadipocytes
摘要
Abstract
[Objective]The biological characteristics of porcine circTEX9 were identified and the regu-lation role of porcine circTEX9 on intramuscular fat deposition was studied.[Method]Based on the previous sequencing data of our research group,circTEX9 was selected as the research object,and its ring structure,stability and subcellular localization were comprehensively identified and analyzed by Sanger sequencing and subcellular localization.At the same time,the regulatory effects of circTEX9 on the proliferation and differentiation of porcine intramuscular preadipocytes were investigated by overexpression method,and the downstream target genes regulated by circTEX9 and the signal path-ways involved were predicted by combining biological information methods.[Result]Porcine circ-TEX9 is a circular RNA molecule composed of exons 7 to 10 of TEX9 gene,which is resistant to RNase R and mainly exists in the cytoplasm.circTEX9 has a stable secondary structure and contains two open reading frames(ORF)and two internal ribosome entry sites(IRES)with translation poten-tial.circTEX9 significantly inhibited the expression levels of proliferative marker genes,and signifi-cantly decreased cell viability.The expression levels of differentiation marker genes were significantly upregulated.Based on the above results and bioinformatics analysis,it was predicted that circTEX9 could absorb ssc-miR-204,ssc-miR-23a and ssc-miR-23b through competing endogenous RNA(ceRNA)network to regulate downstream target genes.These target genes exert biological functions by participating in the adenosine 5 ′-monophosphate-activated protein kinase signaling pathway,cal-cium signaling pathway and mitogen-activated protein kinase signaling pathway,ultimately influencing porcine intramuscular preadipocytes.[Conclusion]circTEX9 has high stability in pig vivo and inhibits the proliferation of intramuscular preadipocytes while promoting lipid deposition,potentially exerting its biological function through ceRNA network.关键词
猪/circTEX9/肌内前体脂肪细胞/细胞增殖和分化/竞争性内源RNAKey words
pigs/circTEX9/intramuscular preadipocytes/cell proliferation and differentiation/com-petitive endogenous RNAs分类
农业科技引用本文复制引用
韦怡林,乔瑞敏,杨峰,韩雪蕾,王冰洁,常乐彬,李新建,王腾飞,李秀领,余彤,原黎伟,昝声远..猪circTEX9生物学特性及其对猪肌内前体脂肪细胞的影响[J].河南农业大学学报,2026,60(1):77-86,10.基金项目
科技创新2030-重大项目(2023ZD04046) （2023ZD04046）
"十四五"国家重点研发计划项目(2021YFD1301202) （2021YFD1301202）
河南省生猪产业技术体系项目(HARS-22-12-G4) （HARS-22-12-G4）
