海南医科大学学报2026，Vol.32Issue(1)：20-27,8.DOI:10.13210/j.cnki.jhmu.20241217.001
青蒿素改善脓毒症小鼠急性肺损伤的剂量相关性研究
Dose-dependent study of artemisinin improving acute lung injury in septic mice
摘要
Abstract
Objective:To investigate the protective effects and dose-dependent relationship of different doses of artemisinin(ART)on acute lung injury in septic mice induced by Gram-positive(G+)and Gram-negative(G-)bacteria.Methods:A total of 90 C57BL/6 mice were randomly divided into the Staphylococcus aureus(G+)and Escherichia coli(G-)groups.Each group included the sham surgery,G+/G-sepsis model,sepsis+2%dimethyl sulfoxide(DMSO)control,and sepsis+different doses of ART(5,10,15,20,25 mg/kg)subgroups.Pro-inflammatory cytokines[tumor necrosis factor-α(TNF-α),interleukin 6(IL-6)]and anti-inflammatory cytokine interleukin 10(IL-10)levels in serum and bronchoalveolar lavage fluid(BALF),lung wet/dry weight ratio(W/D),and H&E staining scores were measured in each experimental group.Results:ART treatment groups dem-onstrated significant dose-dependent protective effects with increasing dosages(5‒25 mg/kg):(1)Inflammatory cytokine levels showed dose-dependent improvement,with the 25 mg/kg dose group significantly reducing TNF-α and IL-6 levels while increas-ing IL-10 level in mice BALF(P<0.05);(2)Pulmonary edema gradually improved with increasing dosages,with high-dose groups(20,25 mg/kg)significantly reducing lung tissue W/D ratios(P<0.05);(3)Pathological damage showed dose-dependent alleviation,most siginficantly in the 25 mg/kg group,characterized by reduced inflammatory cell infiltration and improved alveolar structure.Among all dosage groups,25 mg/kg demonstrated the optimal protective effect.Conclusion:ART ex-hibits significant dose-dependent protective effects against sepsis-induced acute lung injury,with 25 mg/kg being the optimal therapeu-tic dose.The mechanism may be related to dose-dependent inhibition of pro-inflammatory cytokine release,promotion of anti-inflammatory cytokine expression,and improvement of pulmonary edema.These findings provide important evidence for the clinical application of ART in sepsis-related acute lung injury.关键词
青蒿素/脓毒症/肺损伤/炎症因子/剂量依赖性Key words
Artemisinin/Sepsis/Lung injury/Inflammatory factors/Dose-dependency分类
医药卫生引用本文复制引用
赵长甲,张琪,姜波,薛庆,许航,任珊..青蒿素改善脓毒症小鼠急性肺损伤的剂量相关性研究[J].海南医科大学学报,2026,32(1):20-27,8.基金项目
This study was supported by the Central Government Guided Local Science and Technology-Xinjiang Production and Construction Corps Clinical Medical Research Center(Bing Ke Fa 2022-6) （Bing Ke Fa 2022-6）
Corps Guidance Program Project(2023ZD020,2023ZD003) （2023ZD020,2023ZD003）
Eighth Division Shihezi City Young and Middle-aged Science and Technology Innovation Leading Talent Plan Project(2020RC001) 中央引导地方科技-新疆生产建设兵团危重症疾病临床医学研究中心(兵科发2022-6) （2020RC001）
兵团指导性计划项目(2023ZD020,2023ZD003) （2023ZD020,2023ZD003）
八师石河子市中青年科技创新领军人才计划项目(2020RC001) （2020RC001）
