河南农业科学2026，Vol.55Issue(1)：26-39,14.DOI:10.15933/j.cnki.1004-3268.2026.01.003
玉米花粉中响应高温胁迫circRNA的筛选及其功能初探
Screening and Preliminary Exploration of Function of circRNAs Responding to High Temperature Stress in Maize Pollen
摘要
Abstract
Zhengdan 958 with high tolerance to heat and Xianyu 335 with low tolerance to heat were used as research materials.The semi-automatic retractable plastic greenhouse was used for high temperature(HT)stress treatment at flowering stage with normal growth condition as the control(CK).The differentially expressed circRNAs in maize pollen were screened by high-throughput sequencing of circRNA under high temperature stress.GO and KEGG enrichment analysis of the host genes of differentially expressed circRNAs were done.The differentially expressed circRNAs with miRNA binding sites were screened,and their downstream target genes were predicted.The potential circRNA-miRNA-mRNA co-expression regulatory networks which were in response to high temperature stress in maize pollen were constructed.The molecular mechanism of regulating high temperature stress in maize pollen was analyzed from multiple aspects,so as to provide a theoretical basis for improving the heat tolerance of maize varieties.The results showed that a total of 1 843 different circRNAs were identified in different samples of Zhengdan 958 and Xianyu 335.The distribution of circRNAs in maize chromosomes was different.Each circRNA contained different number of exon.Most of them,624 circRNAs contained only one exon.A total of 1563 circRNAs were identified from Zhengdan 958 pollen,305,213 and 356 circRNAs were identified from CK958-1,CK958-2 and CK958-3,respectively,and 222,242 and 225 circRNAs were identified from HT958-1,HT958-2 and HT958-3,respectively.A total of 1 423 circRNAs were identified from Xianyu 335 pollen,272,188 and 229 circRNAs were identified from CK335-1,CK335-2 and CK335-3,respectively,and 259,237 and 238 circRNAs were identified from HT335-1,HT335-2 and HT335-3,respectively.The highest proportions in different samples were all the exon-circRNA.There was no one-to-one correspondence between circRNAs and their host genes,748 circRNA host genes generated one circRNA separately through back splicing mechanism,and 156 circRNA host genes generated two circRNAs separately through back splicing mechanism.Nine differentially expressed circRNAs were screened in the HT958 vs CK958 group,of which two circRNAs were up regulated.Their host genes were significantly enriched in pyrophosphatase activity,nucleoside phosphate metabolic process,glycosylphosphatidylinositol(GPI)anchor metabolic process and other 14 GO items,and two KEGG pathways,including GPI anchor biosynthesis and metabolic pathway.Only one differentially expressed circRNA was screened in the HT335 vs CK335 group,and no significant GO item and KEGG pathway were enriched.Seventeen differentially expressed circRNAs were screened in the HT958 vs HT335 group,of which six circRNAs were up regulated.Their host genes were significantly enriched in endomembrane system,golgi-associated vesicle membrane,membrane protein proteolysis and other 13 GO items,but no significant KEGG pathway.Five circRNAs had miRNA binding sites,which could be used as miRNA sponge island to adsorb miRNAs and indirectly regulate the expression of downstream target genes.circRNA-miRNA-mRNA co-expression regulatory networks,including five circRNAs,five miRNAs from different families and two mRNAs,were constructed.Fifty-four circRNAs which contained the internal ribosomal entry sites(IRES)were screened,which could translate into peptides or proteins and directly act on their target genes.关键词
玉米/花粉/高温胁迫/环状RNA/circRNA来源基因/筛选/功能初探Key words
Maize/Pollen/High temperature stress/circRNA/Host gene of circRNA/Screening/Preliminary exploration of function分类
农业科技引用本文复制引用
李川,张盼盼,张美微,郭涵潇,穆蔚林,牛军,乔江方..玉米花粉中响应高温胁迫circRNA的筛选及其功能初探[J].河南农业科学,2026,55(1):26-39,14.基金项目
河南省科技攻关计划项目(252102110171) （252102110171）
河南省农业科学院自主创新项目(2025ZC20) （2025ZC20）
国家重点研发计划项目(2021YFD1200703-6) （2021YFD1200703-6）
河南省玉米产业技术体系建设项目(HARS-22-02-G2) （HARS-22-02-G2）
河南省农业科学院科技创新团队项目(2024TD31) （2024TD31）
