赣南医科大学学报2025，Vol.45Issue(12)：1125-1130,1137,7.DOI:10.3969/j.issn.2097-7174.2025.12.001
党参炔苷通过PI3K/AKT/GSK-3β信号通路抑制食管鳞状细胞癌KYSE-30细胞增殖及侵袭
Lobetyolin in inhibiting the proliferation and invasion of esophageal squamous cell carcinoma KYSE-30 cells through the PI3K/AKT/GSK-3β signaling pathway
摘要
Abstract
Objective:To investigate the effects of Lobetyolin(LBT)on the proliferation,invasion,and migration of human esophageal squamous cell carcinoma KYSE-30 cells and its underlying mechanism.Methods:Different concentrations(0,10,20,40 µmol·L-1)of LBT were used to treat KYSE-30 cells,with 0 µmol·L-1 as the control group and the other concentrations as the experimental groups.The effects of LBT on the proliferation,colony formation and invasion abilities of KYSE-30 cells were detected by CCK-8 assay,colony formation assay and transwell assay,respectively;the expression of proteins related to cell cycle,epithelial-mesenchymal transition and signaling pathways was detected by western blot.Results:The results of the CCK-8 assay showed that compared with the control group,cell proliferation in the experimental group was inhibited,with a statistically significant difference(P＜0.001).The results of the colony formation assay showed that compared with the control group,the number of cell colonies formed in the experimental group decreased,and the number of colonies decreased with the increase of LBT concentration,with a statistically significant difference(P＜0.001).The results of the transwell assay showed that the invasive and migratory abilities of the experimental group were significantly lower than those of the control group,and the invasive and migratory abilities decreased with the increase of LBT concentration,with a statistically significant difference(P＜0.001).The results of western blot analysis showed that compared with the control group,the proteins of AKT,p-AKTThr308,p-GSK-3βSer9 and CyclinD1 in the experimental group were significantly down-regulated.The epithelial marker E-cadherin in epithelial-mesenchymal transition was significantly up-regulated,while the mesenchymal marker Vimentin and transcription factor Snail were significantly down-regulated.All these changes were concentration-dependent,with statistically significant differences(P＜0.05).Conclusion:LBT may suppress the proliferation and invasion of esophageal squamous cell carcinoma KYSE-30 cells by modulating the PI3K/AKT/GSK-3β signaling pathway.关键词
食管鳞状细胞癌/党参炔苷/PI3K/AKT/GSK-3β/增殖侵袭Key words
Esophageal squamous cell carcinoma/Lobetyolin/PI3K/AKT/GSK-3β/Proliferation and invasion分类
医药卫生引用本文复制引用
黎明春,施华球,黄莉,陈文英,杨颖,郭海亮,张敏鸿,欧阳辛婷,余伟,曾婉晴,衷敬华..党参炔苷通过PI3K/AKT/GSK-3β信号通路抑制食管鳞状细胞癌KYSE-30细胞增殖及侵袭[J].赣南医科大学学报,2025,45(12):1125-1130,1137,7.基金项目
江西省自然科学基金项目(20252BAC240432) （20252BAC240432）
江西省教育厅科学技术研究项目(GJJ2201415) （GJJ2201415）
江西省中医药管理局科技计划项目(2022B961) （2022B961）
江西省卫生健康委员会科技计划项目(202210949) （202210949）
赣州市科技计划重点研发计划重点项目(GZ2024YLJ117) （GZ2024YLJ117）
赣南医科大学第一附属医院博士启动基金(QD075) （QD075）
