河南农业科学2026，Vol.55Issue(1)：52-64,13.DOI:10.15933/j.cnki.1004-3268.2026.01.005
玉米IDL基因家族的全基因组鉴定及在根系发育中的表达分析
Genome-Wide Identification of IDL Gene Family in Maize and Its Expression Analysis during Root Development
摘要
Abstract
The IDL[IDA(inflorescence deficient in abscission)-like]gene family members were identified in maize at the whole-genome level,and their physicochemical properties,phylogenetic evolution,and cis-elements in promoter region were analyzed.The expression patterns of ZmIDL genes in different regions(meristem zone,elongation zone,maturation zone)and tissues(stele,cortex)of the primary root,as well as under hormone and drought treatments were detected using quantitative real-time PCR(qRT-PCR),which would lay a foundation for revealing the role of IDL gene in maize root development.The results showed that seven ZmIDL genes(ZmIDL1-7)were identified,classified into three subgroups and distributed across six chromosomes without collinearity.Their promoter regions contained cis-elements in response to hormones and abiotic stresses.Expression analysis revealed that ZmIDL genes were highly expressed in the root cortex,pericarp|aleurone and silk,and responded to multiple abiotic stresses including drought,low/high temperature,salt and UV.qRT-PCR analysis revealed that ZmIDL1/3/4/6 expression levels were significantly higher in Zhengdan 958 root cortex than those in stele,while ZmIDL2 and ZmIDL7 expression levels were higher in stele;ZmIDL5 did not express.As the germination time extended,ZmIDL1 expression level gradually increased in maturation zone,meristem zone and elongation zone of primary root of Xianyu 335,while ZmIDL6 expression level increased only in the maturation zone of primary root of Xianyu 335,with no significant change in meristem zone and elongation zone of primary root of Zhengdan 958 and Xianyu 335.When auxin transport was blocked in the maturation zone of primary root,ZmIDL1/6 expression level significantly increased,but significantly decreased after transport recovery.Under early drought stress(16 h for Zhengdan 958,8 h for Xianyu 335),ZmIDL6 and ZmIDL1 expression levels increased in the maturation zone of primary root.These findings suggest that ZmIDL6 and ZmIDL1 may synergistically regulate maize root development and respond to drought stress.关键词
玉米/IDL基因/全基因组鉴定/根系发育/进化分析/基因表达/生长素Key words
Maize/IDL genes/Genome-wide identification/Root development/Evolutionary analysis/Gene expression/Auxin分类
农业科技引用本文复制引用
李艳华,李春阳,吴晓林,王伟,刘辉..玉米IDL基因家族的全基因组鉴定及在根系发育中的表达分析[J].河南农业科学,2026,55(1):52-64,13.基金项目
河南省科技攻关项目(242102310550) （242102310550）
河南省高等学校青年骨干教师培养计划项目(2023GGJS167) （2023GGJS167）
