黑龙江水产2026，Vol.45Issue(1)：15-20,6.
膨腹海马嗜环弧菌的分离鉴定、药敏分析及治疗案例
Isolation,identification,drug sensitivity analysis and treatment of vibrio cyclitrophicus in big-belly seahorse(Hippocampus abdominalis)
摘要
Abstract
In March 2024,a disease outbreak occurred in 60-day-old big-belly seahorses(Hippocampus abdomi-nalis)in an indoor industrialized farm in Dalian,Liaoning Province.The diseased seahorses showed symptoms such as slow movement,anorexia,and significant swim bladder enlargement,with continuous sporadic mortality.To identify the pathogen,a dominant bacterium(strain No.12)was isolated from the internal organs(liver,gall-bladder,spleen,and swim bladder)of the diseased seahorses.The strain was identified as Vibrio cyclitrophicus based on colony morphology observation,selective culture medium cultivation,physiological and biochemical tests,and 16S rDNA sequence analysis.The results of the antimicrobial susceptibility test showed that the strain was highly sensitive to enrofloxacin,compound sulfamethoxazole,and chloramphenicol;moderately sensitive to furazoli-done,polymyxin B,erythromycin,norfloxacin,cefazolin,amikacin,and gentamicin;and resistant to penicillin and ampicillin.According to the drug sensitivity results,enrofloxacin powder(aquaculture use,100g:5g)was mixed into the feed and administered at a dose of 0.3g per kilogram of seahorse body weight,once daily for 5 con-secutive days,accompanied by water quality management and environmental regulation.After 4 days of treatment,the diseased seahorses showed significant improvement in condition,with restored feeding activity and vitality,and mortality ceased.This study is the first to report that Vibrio cyclitrophicus can cause swim bladder enlargement and related symptoms in big-belly seahorses,providing a basis for the diagnosis and precise medication of this disease.关键词
膨腹海马(Hippocampus abdominalis)/嗜环弧菌(Vibrio cyclitrophicus)/分离鉴定/药敏试验/恩诺沙星/疾病防治Key words
big-belly seahorse(Hippocampus abdominalis)/Vibrio cyclitrophicus/isolation and identification/drug sensitivity test/enrofloxacin/disease prevention and treatment分类
农业科技引用本文复制引用
冯春明,王淑君..膨腹海马嗜环弧菌的分离鉴定、药敏分析及治疗案例[J].黑龙江水产,2026,45(1):15-20,6.基金项目
辽宁省科技厅重点民生科技类研发项目"北方海马工厂化循环水健康养殖技术研究(2024JH2/102500055)" （2024JH2/102500055）
