黑龙江水产2026，Vol.45Issue(1)：32-37,6.
三种佐剂对美人鱼发光杆菌美人鱼亚种灭活疫苗免疫效果的研究
Study on theimmune efficacy of three adjuvants in an inactivated vaccine a-gainst Photobacterium damselae subsp.damselae in Zebrafish(Danio rerio)
摘要
Abstract
In recent years,infections caused by Photobacterium damselae subsp.damselae in aquatic animals have been increasing.Reliance on antibiotic treatment leads to drug resistance and food safety concerns.The combina-tion of inactivated vaccines with adjuvants can significantly enhance immune efficacy.This study aimed to evaluate the enhancing effects of three adjuvants(Astragalus polysaccharides,Freund's complete adjuvant,and 4%alumi-num hydroxide gel)on an inactivated P.damselae subsp.damselae vaccine.The optimal formaldehyde inactivation condition was first determined to be 0.4%(v/v)for 8 h.Zebrafish(Danio rerio)were then immunized via intrap-eritoneal injection and challenged.Results showed that the relative percent survival(RPS)of the inactivated vac-cine alone was 58.3%,while RPS values for vaccine groups combined with Astragalus polysaccharides,Freund's complete adjuvant,and aluminum hydroxide gel were 74%,45.8%,and 62.5%,respectively.Further monitoring of dynamic changes in non-specific immune indicators(ACP,AKP,CAT,SOD,MDA,LZM)revealed that the Astragalus polysaccharide-adjuvanted group significantly(P<0.05)and sustainably enhanced the host's immune response with good safety.In conclusion,Astragalus polysaccharide is the optimal adjuvant for enhancing the im-mune efficacy of the inactivated P.damselae subsp.damselae vaccine,providing an experimental basis for develo-ping efficient and safe aquaculture vaccines.关键词
黄芪多糖/灭活疫苗/佐剂/斑马鱼(Danio rerio)/美人鱼发光杆菌美人鱼亚种(Photobacterium dam-selae subsp.damselae)Key words
Astragalus polysaccharides/inactivated vaccine/adjuvant/Danio rerio/Photobacterium damselae sub-sp.damselae分类
农业科技引用本文复制引用
程浩森,周子纯,孟志,张春雨,李文婧,贾旭颖,周文礼,邵蓬..三种佐剂对美人鱼发光杆菌美人鱼亚种灭活疫苗免疫效果的研究[J].黑龙江水产,2026,45(1):32-37,6.基金项目
国家自然科学基金(No.32303038) （No.32303038）
