湖南中医药大学学报2026，Vol.46Issue(1)：7-13,7.DOI:10.3969/j.issn.1674-070X.2026.01.002
基于Wnt/β-catenin信号通路探讨温阳振衰颗粒对慢性心力衰竭大鼠心室重构的影响
Effects of Wenyang Zhenshuai Granules on ventricular remodeling in rats with chronic heart failure via the Wnt/β-catenin signaling pathway
摘要
Abstract
Objective To explore the effects of Wenyang Zhenshuai Granules(WZG)on ventricular remodeling in rats with chronic heart failure(CHF)based on the regulation of Wnt/β-catenin signaling pathway.Methods A CHF rat model was established via intraperitoneal injection of doxorubicin(DOX).After successful modeling,rats were randomly assigned to model group(CHF group),WZG low-dose group(WZG-L group,0.72 g/kg),WZG medium-dose group(WZG-M group,1.44 g/kg),WZG high-dose group(WZG-H group,2.88 g/kg),and enalapril group(ENP group,1.8 mg/kg),with ten rats per group.An additional ten rats served as normal control group(NC group).The NC and CHF groups received equal volumes of distilled water,while the remaining groups received corresponding drugs by gastric gavage at their respective doses for four consecutive weeks.Echocardiography was used to evaluate cardiac function.Serum N-terminal pro-brain natriuretic peptide(NT-proBNP)levels were measured by ELISA.Left ventricular mass index(LVMI)was calculated to evaluate ventricular remodeling.Myocardial tissue pathology and collagen volume fraction(CVF)were examined using Masson's trichrome staining.Western blot was performed to determine the Dishevelled-1(DVL1)and β-catenin protein expressions in myocardial tissues.Results Compared with the NC group,rats in the CHF group exhibited increased left ventricular internal diameter at end-diastole(LVIDd),left ventricular internal diameter at end-systole(LVIDs),and LVMI(P<0.05,P<0.01),decreased ejection fraction(EF),and left ventricular shortening fraction(FS)(P<0.01),disorganized myocardial fiber arrangement,increased CVF(P<0.01),higher serum NT-proBNP levels(P<0.01),and up-regulated expressions of DVL1 and β-catenin in the myocardial tissues(P<0.01).Compared with the CHF group,the WZG-L,WZG-M,and WZG-H groups exhibited reduced LVIDd,LVIDs,and LVMI(P<0.01),increased EF and FS(P<0.01),alleviated myocardial pathological injury with decreased CVF(P<0.05,P<0.01),lower serum NT-proBNP levels(P<0.01),and downregulated DVL1 and β-catenin expressions in the myocardial tissues(P<0.05).Conclusion WZG can effectively ameliorate myocardial injury in rats with DOX-induced CHF,mitigate myocardial fibrosis,and inhibit ventricular remodeling.Its mechanism of action may be associated with the suppression of the abnormal expression of key proteins in the Wnt/β-catenin signaling pathway.关键词
慢性心力衰竭/温阳振衰颗粒/心肌纤维化/心室重构/Wnt/β-catenin信号通路Key words
chronic heart failure/Wenyang Zhenshuai Granules/myocardial fibrosis/ventricular remodeling/Wnt/β-catenin signaling pathway分类
医药卫生引用本文复制引用
彭丽琪,欧长莹,王贤颖,陈梦婷,魏子涵,陈新宇,唐燕萍..基于Wnt/β-catenin信号通路探讨温阳振衰颗粒对慢性心力衰竭大鼠心室重构的影响[J].湖南中医药大学学报,2026,46(1):7-13,7.基金项目
湖南省自然科学基金高校联合基金项目(2025JJ90051) （2025JJ90051）
湖南省中医药科研课题(B2023026) （B2023026）
湖南省卫生健康委员会科研计划项目(202203015711) （202203015711）
国家级大学生创新训练计划项目(S202410541043) （S202410541043）
湖南省大学生创新创业训练计划项目(2024-2864) （2024-2864）
湖南中医药大学本科生科研创新基金项目(2023BKS036,2024BKS139) （2023BKS036,2024BKS139）
湖南省研究生科研创新项目(CX20230806). （CX20230806）
