国际神经病学神经外科学杂志2025，Vol.52Issue(6)：25-32,8.DOI:10.16636/j.cnki.jinn.1673-2642.2025.06.004
肌萎缩侧索硬化液体生物标志物与临床特征的相关性
Correlation between fluid biomarkers and clinical features in amyotrophic lateral sclerosis
摘要
Abstract
Objective To investigate the correlation between fluid biomarkers and clinical features in amyotrophic lateral sclerosis(ALS).Methods A total of 27 ALS patients who were treated in Shiyan People's Hospital from January 2019 to February 2023 were enrolled as ALS group,and 28 volunteers without central nervous system disorders were enrolled as control group.Sandwich ELISA was used to measure the levels of vascular endothelial growth factor(VEGF),VEGF receptor 2(VEGFR2),optineurin(OPTN),TAR DNA-binding protein 43(TDP-43),angiopoietin(ANG),and monocyte chemoattractant protein 1(MCP-1)in plasma and cerebrospinal fluid(CSF).Related clinical data were collected at the time of sample collection,and clinical features and fluid biomarkers were compared between the two groups.Results Compared with the control group,the ALS group had significantly lower levels of VEGF,VEGFR2,OPTN,TDP-43,and ANG and a significantly higher level of MCP-1 in plasma and CSF(P<0.05).After univariate linear regression analysis and correction,plasma VEGF was negatively correlated with disease progression rate(P<0.05),and plasma VEGF,VEGFR2,and OPTN were positively correlated with the score of ALS Functional Rating Scale-Revised(ALSFRS-R)(P<0.05);in addition,VEGF in CSF was negatively correlated with disease progression rate(P<0.05),and VEGF and VEGFR2 in CSF were positively correlated with ALSFRS-R score(P<0.05).After correction,plasma ANG was negatively correlated with disease progression rate(P<0.05).After univariate linear regression analysis and correction,TDP-43 in plasma and CSF was positively correlated with age in ALS patients(P<0.05),and plasma MCP-1 was positively correlated with disease progression rate and was negatively correlated with ALSFRS-R score(P<0.05);the univariate regression analysis showed that MCP-1 in CSF was also positively correlated with disease progression rate(P<0.05).The Spearman rank correlation analysis showed positive correlations between plasma biomarkers(P<0.05);as for the CSF biomarkers,VEGF was positively correlated with VEGFR2(P<0.05),and TDP-43 was positively correlated with other biomarkers(P<0.05).Conclusions Six fluid biomarkers are associated with age,disease severity,and disease progression in ALS patients.关键词
肌萎缩侧索硬化/血管内皮生长因子/血管内皮生长因子受体2/视神经蛋白/反式激活反应DNA结合蛋白43/血管生成素/单核细胞趋化蛋白-1Key words
amyotrophic lateral sclerosis/vascular endothelial growth factor/vascular endothelial growth factor receptor 2/optineurin/TAR DNA-binding protein 43/angiopoietin/monocyte chemoattractant protein 1分类
医药卫生引用本文复制引用
汤锦秀,樊露,隋晓菲,高根善..肌萎缩侧索硬化液体生物标志物与临床特征的相关性[J].国际神经病学神经外科学杂志,2025,52(6):25-32,8.基金项目
湖北省卫生健康委科研项目(WJ2023Ml96). （WJ2023Ml96）
