经济动物学报2026，Vol.30Issue(1)：23-33,11.DOI:10.13326/j.jea.2026.1923
猪流行性腹泻病毒结构蛋白对细胞极性分子Par6表达的影响
Effects of Porcine Epidemic Diarrhea Virus Structural Proteins on the Expression of Cellular Polar Molecule Par6
摘要
Abstract
Tight junctions(TJs)are the major junctional structures among intestinal epithelial cells,which can resist the invasion of external pathogens and selectively regulate the entry of small molecules and ions into the body.In intestinal epithelial cells,polarity complexes can interact with tight junctions to maintain cell polarity.To investigate the effects of porcine epidemic diarrhea virus(PEDV)structural proteins on the expression of the cell polarity molecule Par6,eukaryotic expression plasmids carrying PEDV N,S1,S2,M and E genes were constructed.Using Lipofectamine 3000 transfection reagent,time-course and dose-response groups were set up for transfection into porcine jejunum epithelial cell line(IPEC-J2).Specifically,in the time-course group,cells were transfected with a fixed dose of 5 μL and samples were collected at 24,36 and 48 hours post-transfection;in the dose-response group,cells were transfected with 1,3 and 5 μL of the reagent and samples were collected at 24 hours post-transfection.The expression levels of Par6 mRNA and protein in the cells were then detected.The results showed that transfection with eukaryotic expression plasmids carrying PEDV N,S1,S2,M and E proteins all up-regulated the expression of Par6 mRNA and protein in the cells,with the N protein exerting the most significant up-regulatory effect.This study provides a reference for revealing the pathogenic mechanism of PEDV.关键词
猪流行性腹泻病毒/结构蛋白/细胞极性/Par6Key words
porcine epidemic diarrhea virus/structural protein/cell polarity/Par6分类
农业科技引用本文复制引用
张若兰,赵石卉,刘岸,曾小容,赵涵,鲍迪,裴志花,王开,胡桂学..猪流行性腹泻病毒结构蛋白对细胞极性分子Par6表达的影响[J].经济动物学报,2026,30(1):23-33,11.基金项目
国家自然科学基金项目(32072857),吉林省科技发展计划项目(20230101257JC,20240101219JC),长春市科技发展计划项目(23CZ05) （32072857）
