耕作与栽培2025，Vol.45Issue(11)：16-21,6.
EMS诱变对油菜种子萌发及幼苗表型特征的影响
Effect of EMS Mutagenesis on Seed Germination and Seedling Phenotypic Characteristics of Oilseed Rape
摘要
Abstract
To create intermediate oilseed rape materials and investigate the effects of ethyl methanesulfonate(EMS)mutagen-esis on seed germination while determining the optimal mutagenesis treatment combination,seeds of Yunyouza No.15 were used as experimental material.Twelve treatment combinations were applied,including five EMS buffer solution concentra-tions(0.20%,0.40%,0.60%,0.80%,and 1.00%)and two exposure durations(2 h and 4 h).The results showed that as the EMS concentration increased,seed germination and seedling vigor of Yunyouza No.15 were significantly inhibited,with lon-ger exposure times amplifying this inhibitory effect.The concentration of 1.00%EMS with a 4 h exposure was identified as the half-lethal dose,representing the optimal mutagenesis treatment combination for oilseed rape seeds.Phenotypic evalua-tion of seedlings revealed variant phenotypes such as dwarfing,slender seedlings,leaf yellowing,purple or brown necrotic spots on leaves,and cotyledon malformation.关键词
EMS诱变/油菜/种子萌发/幼苗活力/表型评价Key words
EMS mutagenesis/Brassica napus L./seed germination/seedling vigor/phenotypic evaluation分类
农业科技引用本文复制引用
蓝新隆,林武,夏春英,陈松亮,萧涵,林国强,张玉梅..EMS诱变对油菜种子萌发及幼苗表型特征的影响[J].耕作与栽培,2025,45(11):16-21,6.基金项目
福建省农业科学院自由探索科技创新项目(ZYTS202406) （ZYTS202406）
福建省财政专项(CXTD2021011-2) （CXTD2021011-2）
