吉林农业大学学报2026，Vol.48Issue(1)：81-86,6.DOI:10.13327/j.jjlau.2024.20357
菜豆原生质体的融合条件研究
Fusion Conditions of Protoplast from Common Bean
摘要
Abstract
Protoplast fusion is an effective means to create and breed new varieties of strictly self-pollinated crops.PEG-high Ca2+-high pH fusion method is an ideal method to promote protoplast fu-sion with high efficiency.To establish a protoplast fusion system for common bean,the protoplast fu-sion of common bean was induced by PEG-high Ca2+-high pH method,and the suitable fusion meth-ods and conditions for bean protoplasts were studied.The results showed that the optimal conditions for protoplast fusion in beans are as follows:a protoplast density of 1.3×105/mL,initial induction of fusion with 35%PEG(MW=6 000),followed by the sequential addition of 20 µL of high Ca2+-high pH solution four times,incubation at room temperature for 60-90 min,and finally washing to pro-mote fusion with with 80 µL of high Ca2+-high pH solution for 2-3 times,whcih can achieve a fusion rate of up to 13%.关键词
菜豆/原生质体/PEG-高Ca2+-高pH融合/体细胞杂交Key words
Phaseolus vulgaris L./protoplast/PEG-high Ca2+-high pH fusion/somatic cell hybrid-ization分类
农业科技引用本文复制引用
韩玉珠,张金鹏,乔宏宇,张婧颖,张广臣..菜豆原生质体的融合条件研究[J].吉林农业大学学报,2026,48(1):81-86,6.基金项目
吉林省科技发展计划项目(20220202099NC),吉林农业科技学院项目[合字第(2023)702号] （20220202099NC）
