中国牛业科学2025，Vol.51Issue(6)：15-23,9.DOI:10.26951/j.cnki.ccs.2025.06.004
郏县红牛背最长肌差异表达lncRNA的筛选及其功能分析
Screening and Functional Analysis of Differentially Expressed lncRNAs in the Longissimus Dorsi Muscle of Jiaxian Red Cattle
摘要
Abstract
The purpose of this study was to explore the important role of long non-coding RNA(lncRNA)in the deposition of intramuscular fat(IMF)in bovine and related molecular mechanisms.Tran-scriptome sequencing technology was used to analyze the longissimus dorsi muscle samples of A2 and A4 grade Jiaxian Red Cattle,and the differentially expressed lncRNAs were screened,and their target genes were predicted and GO and KEGG enrichment were analyzed.The results showed that 190 differentially ex-pressed lncRNAs were screened in the A2 and A4 groups,of which 94 lncRNAs were significantly up-regu-lated and 96 lncRNAs were significantly down-regulated in the A4 group compared with the A2 group.These differentially expressed lncRNAs regulate 7 and 175 target genes respectively through cis-and trans-regulation.Enrichment analysis showed that the trans target genes were significantly enriched in lipid me-tabolism,fatty acid metabolism and propionate metabolism pathways(P≤0.05).These results suggest that differentially expressed lncRNAs may participate in the regulation of bovine intramuscular fat deposition through trans-regulatory target genes.关键词
郏县红牛/长链非编码RNA(lncRNA)/肌内脂肪/转录组测序Key words
Jiaxian Red Cattle/long non-coding RNA(lncRNA)/intramuscular fat/transcriptome se-quencing分类
农业科技引用本文复制引用
柳丽,邱亮,祁兴山,昝林森,仝林,杨武才..郏县红牛背最长肌差异表达lncRNA的筛选及其功能分析[J].中国牛业科学,2025,51(6):15-23,9.基金项目
国家肉牛牦牛产业技术体系建设项目(CARS-37) （CARS-37）
陕西省重点研发计划"两链"融合重点专项揭榜挂帅项目(2022GD-TSLD-46-0104) （2022GD-TSLD-46-0104）
