寄生虫与医学昆虫学报2025，Vol.32Issue(4)：231-237,7.DOI:10.3969/j.issn.1005-0507.2025.04.006
犬源血红扇头蜱形态学及分子生物学鉴定
MORPHOLOGICAL AND MOLECULAR IDENTIFICATION OF RHIPICEPHALUS SANGUINEUS FROM DOGS
苗壮 1李熙 2李闻静 1周芳芳 1孙淑雯 1程天印 1李芬1
作者信息
- 1. 湖南农业大学动物医学院,长沙 410128
- 2. 长沙市农业科学研究院,长沙 410100
- 折叠
摘要
Abstract
Objective The aims of this study were to identify tick species infesting police dogs and explore their phylogenetic relationships.Methods Ticks were collected from a police dog base in Hunan Province and their diagnostic morphological characteristics and phylogenetic relationships were investigated using PCR targeting the 16S rDNA,cytochrome C oxidase subunit 1(cox1),and NADH dehydrogenase subunit V(nad5)genes.Results Adult ticks were oval shaped with a broad and short hexagonal basis capitulum.The females had a scutum occupying the first 1/3 of the body,a U-shaped genital groove,and a comma-shaped stigmal plate,and their coxae Ⅱ to Ⅳ each had short and coarse outer spurs that gradually decreased in size according to the segmental order.Males had an elongated scutum,a comma-shaped stigmal plate,and a subtriangular adanal plate.The product sizes of the amplified 16S rDNA,cox1,and nad5 gene sequences from the ticks in this study were approximately 405,772,and 262 bp,respectively.The phylogenetic tree based on these sequences showed that this tick species clustered with Rhipicephalus sanguineus of other tropical lineages.Conclusions The tick species collected from police dogs in China is Rh.sanguineus,which belongs to the tropical lineage.关键词
血红扇头蜱/形态学/16S rDNA/cox1/nad5/系统发育树Key words
Rhipicephalus sanguineus/morphology/16S rDNA/cox1/nad5/phylogenetic tree分类
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苗壮,李熙,李闻静,周芳芳,孙淑雯,程天印,李芬..犬源血红扇头蜱形态学及分子生物学鉴定[J].寄生虫与医学昆虫学报,2025,32(4):231-237,7.
