菌物研究2025，Vol.23Issue(6)：437-448,12.DOI:10.13341/j.jfr.2025.1890
布莱克韦尔虫草的药物化学基础与抗乳腺癌潜力研究
Pharmacochemical Analysis and Potential Roles of Cordyceps blackwelliae on Anti-Breast Cancer
摘要
Abstract
For in-depth understanding of the bioactive components and pharmacological properties of Cordyceps blackwelliae,advanced methods by using the ultra-high-performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)in combination with the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)were employed in this study.Preliminary screening via the TCMSP database identified a total of 144 potential bioactive components,in which eleven characteristic components were finally confirmed to contain significant pharmacological activities on the basis of both oral bioavailability(OB≥30％)and drug-likeness(DL≥0.18).Association analysis of targets and diseases revealed a strong correlation between the C.blackwelliae's bioactive components and the human breast cancer,leading to identification of 45 intersection targets in total.Enrichment analysis of KEGG pathway demonstrated that genes of these targets were significantly enriched in a multiple number of pathways showing relations to cancers,including the apoptosis regulation pathway,IL-17-mediated inflammatory signaling pathway,and the PI3K-Akt-mediated cell proliferation and survival signaling pathway.In vitro experiments confirmed that the treatment of C.blackwelliae remarkedly altered the morphology of triple-negative breast cancer MDA-MB-231 cells and inhibited the cell viability,migration and invasion capabilities as well.This study elucidated the multi-component,multi-target and multi-pathway synergistic mechanism of C.blackwelliae against breast cancer that should provide a theoretical and practical basis for future development of novel and natural anti-breast cancer drugs.关键词
布莱克韦尔虫草/网络药理学/活性成分/乳腺癌/MDA-MB-231细胞Key words
Cordyceps blackwelliae/network pharmacology/bioactive component/breast cancer/MDA-MB-231 cell分类
农业科技引用本文复制引用
李佳妮,张永杰..布莱克韦尔虫草的药物化学基础与抗乳腺癌潜力研究[J].菌物研究,2025,23(6):437-448,12.基金项目
国家自然科学基金项目(31872162 （）
32370014),山西省"三晋英才"计划项目,山西省基础研究计划项目(202203021221036) （202203021221036）
