菌物研究2025，Vol.23Issue(6)：467-475,9.DOI:10.13341/j.jfr.2023.1703
松杉灵芝的生物学特性及林下栽培研究
Analysis of Biological Characteristics and Under-Forest Cultivation of Ganoderma tsugae
摘要
Abstract
In order to fully exploit and utilize macrofungal resources,this study aims to investigate the feasibility of under-forest cultivation of Ganoderma in the northern Shaanxi area of China.A wild Ganoderma strain,collected from Mohe of Heilongjiang Province,was isolated and purified.Morphological characterization and molecular biological analysis identified this strain as belonging to the species of Ganoderma tsugae.Effects of the different carbon sources,Tsuga sawdust extract concentration,pH value and temperature of culture medium on its mycelial growth of G.tsugae were then examined.Single-factor experiments revealed that the optimal conditions of cultivation for mycelial growth of mother-strain were to use glucose as the carbon source,a concentration of 60 g/L of Tsuga sawdust extract,a temperature of 25℃ and a value of pH 5.0.Selection of three optimal levels to optimize above factors by using the response surface methodology(RSM),furthermore,suggested the ideal conditions for mycelia growth of G.tsugae that included a concentration of 61 g/L Tsuga sawdust extract,26℃,and pH 5.0.Under these conditions,the mycelia morphologically grew dense and revealed a growth rate reaching to 8.15 mm/d,more than two-fold higher than that of the control group.Comparative analysis revealed a lower yield but significantly higher bioactive compound contents of G.tsugae fruitbody cultivated under the natural forests than that within the greenhouse,in which the content of triterpenes and polysaccharides detected from the samples grown under-forest was 49.94％and 50.91％higher than that grown within greenhouse,respectively.These findings should provide a scientific basis for optimizing culture conditions of mother-strain and under-forest cultivation of G.tsugae that,furthermore,give valuable references for the future research and development of other Ganoderma species.关键词
松杉灵芝/鉴定/响应面法/优化培养/林下栽培Key words
Ganoderma tsugae/identification/response surface methodology/optimization cultiva-tion/under-forest cultivation分类
农业科技引用本文复制引用
罗丽媛,荆杨梦杰,马泉,贺晓龙,高小朋,李旭,刘月芹..松杉灵芝的生物学特性及林下栽培研究[J].菌物研究,2025,23(6):467-475,9.基金项目
国家自然科学基金项目(22268045),陕西省博士科学研究基金项目(YDBK2015-08),陕西省科技厅项目(2025NC-YBXM-078),陕西省教育厅项目(24JC088) （22268045）
